FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences270502
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC9310.34417490443693577No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG7920.2927889627433439No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT7460.27578354319006887No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA7100.2624749539744623No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC6910.25545097633289215No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT5810.21478584261853886No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA5610.20739218194320191No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA5540.20480440070683395No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT5470.202216619470466No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA5470.202216619470466No Hit
GCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCG5400.19962883823409805No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC5210.19260486059252796No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG5100.18853834722109264No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG4930.18225373564705621No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT4750.17559944103925296No Hit
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC4710.17412070890418554No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA4600.17005419553275022No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA4560.16857546339768284No Hit
GCTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT4540.16783609733014912No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA4510.1667270482288486No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAA4290.15859402148597793No Hit
GCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCG4170.15415782508077575No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC4100.1515700438444078No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCT4060.15009131170934042No Hit
GTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGG3990.14750353047297246No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA3990.14750353047297246No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTACTATGTCTGG3990.14750353047297246No Hit
GGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC3820.14121891889893606No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGC3760.13900082069633496No Hit
CCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCA3630.13419494125736592No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC3550.13123747698723115No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGTATGCTCTCACTC3500.1293890618183969No Hit
GATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGA3420.1264315975482621No Hit
CCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGA3420.1264315975482621No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGG3350.12384381631189419No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAG3280.12125603507552624No Hit
CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTG3220.11903793687292515No Hit
GTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGC3210.11866825383915831No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGT3190.11792888777162462No Hit
GTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTT3140.11608047260279036No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTG3090.11423205743395612No Hit
CTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCT3060.11312300833265557No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCT3050.11275332529888872No Hit
GGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG3040.11238364226512189No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT3030.11201395923135504No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAACTTACCA2960.10942617799498709No Hit
GACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAAT2930.10831712889368655No Hit
GAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCA2860.10572934765731862No Hit
GCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATA2850.10535966462355177No Hit
ACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGA2820.10425061552225123No Hit
ACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTT2770.10240220035341698No Hit
GCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTA2770.10240220035341698No Hit
GCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTT2750.10166283428588328No Hit
CCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAGAGACGGGGGA2750.10166283428588328No Hit
CCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAG2730.10092346821834958No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACCTGT2730.10092346821834958No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGCTGT2710.10018410215081588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)