FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228791
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC7110.31076397235905256No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG6200.27098968053813305No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT5800.25350647534212445No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC5340.23340078936671457No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA4940.215917584170706No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA4710.20586474118300108No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA4700.2054276610531009No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA4570.1997456193643981No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT4560.19930853923449784No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA4480.19581189819529612No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC4460.1949377379354957No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT4370.19100401676639378No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAA4280.18707029559729185No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT4240.185321975077691No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG4110.1796399333889882No Hit
GCTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT4090.1787657731291878No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGC4000.17483205196008586No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA3970.17352081157038518No Hit
CCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGA3710.1621567281929796No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC3670.16040840767337877No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC3650.15953424741357833No Hit
GTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGG3310.14467352299697103No Hit
GGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC3220.1407398018278691No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG3210.14030272169796887No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT3140.13724316078866738No Hit
CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTG3020.13199819922986483No Hit
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC2970.12981279858036374No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGG2910.12719031780096243No Hit
GCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCG2900.12675323767106222No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCT2850.12456783702156117No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA2840.12413075689166095No Hit
GCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCG2810.12281951650196031No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA2790.12194535624215988No Hit
GTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTT2780.12150827611225964No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGTATGCTCTCACTC2780.12150827611225964No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTACTATGTCTGG2760.12063411585245924No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGT2750.12019703572255902No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTG2730.11932287546275859No Hit
TCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTA2590.1132037536441556No Hit
CTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAAGCGGGGCTCTGC2580.11276667351425536No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAG2540.1110183529946545No Hit
CCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTG2530.1105812728647543No Hit
ACTCTGGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACT2520.11014419273485407No Hit
CCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCA2500.10927003247505364No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCT2500.10927003247505364No Hit
CCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGG2490.10883295234515343No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTAT2410.10533631130595171No Hit
GCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTA2410.10533631130595171No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGCTGT2380.10402507091625107No Hit
GACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAAT2370.10358799078635085No Hit
GAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCA2360.10315091065645064No Hit
ACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGA2360.10315091065645064No Hit
TCTCAGAAGATCAAGGTCGGTCGGCGGTGCAACCCTCAAGGGGATCCCGCC2290.10009134974714914No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)