FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391157
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT90552.3149272542738593Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC74881.914320848150487Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG39861.0190281651613036Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT26400.6749208118479281Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACG23440.5992478723377058No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA13290.3397612723279911Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCATCACAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACG12880.3292795475985346No Hit
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11960.305759579912925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG11680.29860132887817425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA10900.27866048670994Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT8890.22727447035333637Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACGA8760.2239509966586307No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG7410.18943800059822527No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC6940.1774223649327508No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA6580.16821889931664266Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAA6300.16106064828189193Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTATA5530.14137545793632736Clontech SMART CDS Primer II A (96% over 26bp)
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT5320.1360067696602643No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA5250.1342172069015766No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC5210.13319459961089794No Hit
GTGATGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT5000.12782591133483487Clontech SMART CDS Primer II A (96% over 26bp)
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA4940.12629200039881683No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA4850.12399113399478982No Hit
GTATCATCACAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACGA4470.11427636473334236No Hit
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT4400.11248680197465469Clontech SMART CDS Primer II A (96% over 26bp)
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT4280.10941898010261863No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC4070.10405029182655558No Hit
ACGAGACAGGGTTGGACCTGTCTCTTATACACATCTCCGAGCCCACGAGAC4050.10353898818121623No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA4020.10277203271320723No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGC3980.10174942542252854Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT3970.10149377359985887No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)