FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391157
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC75861.9393747267721146Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT60521.5472048307968411Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT32860.8400718892925348Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG31450.8040249822961113Clontech Universal Primer Mix Long (96% over 25bp)
GTCCAACCCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA23460.5997591759830452Clontech Universal Primer Mix Long (95% over 23bp)
GTCCAACCCTGCGTTGATACCCTGTCTCTTATACACATCTGACGCTGCCGA21910.5601331434692464No Hit
GTCCAACCCTGTGATGATACCACTGCTTCCATGTACTCTGCGTTGATACCA16420.41978029282359774No Hit
GTCCAACCCTGTGATGATACCCTGTCTCTTATACACATCTGACGCTGCCGA12520.3200760819824265No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA11030.28198396040464574Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT9220.23571098050143546Clontech SMART CDS Primer II A (96% over 26bp)
GTCCAACCCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGAC9100.23264315862939947No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG7050.18023453498211714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC7050.18023453498211714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA6880.17588845399673275Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC6580.16821889931664266No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTATA6430.16438412197659763Clontech SMART CDS Primer II A (96% over 26bp)
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG6230.15927108552320424No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC5520.14111980611365768No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA5390.13779633241895198Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGT5340.13651807330560364Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAA5140.13140503685221022Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGATGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT5000.12782591133483487Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA4980.1273146076894955No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT4960.12680330404415618No Hit
GTCCAACCCTGTGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA4950.1265476522214865No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA4670.11938940118673576No Hit
GTCCAACCCTGTGATGATACCTGTCTCTTATACACATCTGACGCTGCCGAC4400.11248680197465469No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA4220.10788506916660062No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG4050.10353898818121623No Hit
GTCCAACCCTGTCTCGTCTGTCTCTTATACACATCTGACGCTGCCGACGAT4040.10328333635854657No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC3960.1012381217771892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)