FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325371
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC25930.7969364202710136Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT23640.7265552246512443Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG11180.34360775852795733Clontech Universal Primer Mix Long (96% over 25bp)
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC8110.2492539285922839No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT7800.23972634315903998Clontech SMART CDS Primer II A (96% over 26bp)
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG7130.21913446496460964No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC5860.1801020988348685No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA5850.17979475736928No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA5700.1751846353854523No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA5510.16934514753927055No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT5380.16534970848661987No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT5000.1536707327942564No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC4770.14660187908572062No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT4620.1419917571018929No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA4300.13215683020306052No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT4110.12631734235687878No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG4030.12385861063217066No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA3880.11924848864834296No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA3660.11248697640539569No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3580.11002824468068757Clontech SMART CDS Primer II A (96% over 26bp)
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC3480.10695483002480247No Hit
CCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCA3300.10142268364420923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)