FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302892
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC29520.9746048096351175Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT25400.8385827291575875Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG12290.4057551866671949Clontech Universal Primer Mix Long (96% over 25bp)
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT10680.35260092706311164No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC10320.34071550255536626No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG9270.30604968107444236No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT8600.2839295854628052No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT8550.2822788320589517Clontech SMART CDS Primer II A (96% over 26bp)
GCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA8180.2700632568704357No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA8060.26610144870118724No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA7750.2558667775972954No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA7650.25256527078958835No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT7600.25091451738573484No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT7400.2443115037703208No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG7260.23968939423953092No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC7210.2380386408356774No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC7010.23143562722026334No Hit
GTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGG6470.21360749045864535No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG6350.20964568228939687No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAA6290.20766477820477267No Hit
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC6240.20601402480091915No Hit
GCTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT6180.20403312071629492No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA6180.20403312071629492No Hit
GAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCA6030.19908086050473436No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA6010.19842055914319295No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC5980.19743010710088085No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTACTATGTCTGG5580.18422407987005268No Hit
CCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGA5490.18125272374311638No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGC5310.1753100114892437No Hit
GACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAAT5260.17365925808539018No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT5230.17266880604307808No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA5180.17101805263922454No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGG5170.17068790195845382No Hit
CCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCA4700.15517081996223075No Hit
GTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTT4630.15285976519683583No Hit
GGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC4610.15219946383529442No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTAT4600.1518693131545237No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC4580.15120901179298232No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAG4520.1492281077083581No Hit
CTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCT4480.14790750498527527No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCT4480.14790750498527527No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGT4360.14394569681602684No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGCTGT4320.14262509409294402No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA4320.14262509409294402No Hit
GCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCG4280.1413044913698612No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA4280.1413044913698612Clontech Universal Primer Mix Long (96% over 25bp)
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTG4250.1403140393275491No Hit
GCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCACT4210.13899343660446628No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACCTGT4210.13899343660446628No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCT4190.13833313524292487No Hit
CTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAAGCGGGGCTCTGC4180.13800298456215415No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCT4170.13767283388138346No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG4160.13734268320061277Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG4130.13635223115830064No Hit
GATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC4060.13404117639290572No Hit
TGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGA4000.1320602723082815No Hit
CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTG3990.1317301216275108No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3960.1307396695851987Clontech SMART CDS Primer II A (96% over 26bp)
GCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCT3920.12941906686211585No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA3880.12809846413903306Clontech Universal Primer Mix Long (96% over 25bp)
ACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTC3870.12776831345826234No Hit
GCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCG3860.12743816277749165No Hit
ACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGG3850.12710801209672096No Hit
CCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAA3790.1251271080120967No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTTC3720.1228160532467018No Hit
TCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTA3720.1228160532467018No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAACTTACCA3700.1221557518851604No Hit
GCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATA3680.12149545052361897No Hit
GTTATTGCCTCAAACTTCCTTGGCCTAAGCGGCCATAGTCCCTCTAAGAAG3670.12116529984284828No Hit
CGCTTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACG3660.12083514916207756No Hit
GCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTA3640.12017484780053617No Hit
ACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGA3600.11885424507745335No Hit
GACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTG3590.11852409439668266No Hit
ACTCTGGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACT3590.11852409439668266No Hit
GCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTT3580.11819394371591194No Hit
CCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAG3550.11720349167359982No Hit
GAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGAT3550.11720349167359982No Hit
CAGGATAGCTGGAGCCCACGTGCGAGTTCTATCGGGTAAAGCCAATGATTA3550.11720349167359982No Hit
GATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGA3520.11621303963128772No Hit
GCTGATGACTCGCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCA3520.11621303963128772No Hit
GCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTT3460.1142321355466635No Hit
GTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGC3440.1135718341851221No Hit
TCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCACG3390.11192108078126857No Hit
CCAAGGAGTCTGACATGTGTGCGAGTCAACGGGCCAGTAAACCCGTAAGGC3390.11192108078126857No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGA3350.11060047805818575No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGT3340.11027032737741504No Hit
CCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTG3300.10894972465433225No Hit
GTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGA3250.10729897125047873No Hit
GAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGAT3240.10696882056970801No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGTATGCTCTCACTC3230.10663866988893732No Hit
CCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGAATTTC3220.1063085192081666No Hit
GGGCAGAGCCCCGCTTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGT3210.1059783685273959No Hit
CCGCGGCCAGCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTG3210.1059783685273959No Hit
CCAAGGGAACGGGCTTGGCAGAATCAGCGGGGAAAGAAGACCCTGTTGAGC3150.10399746444277169No Hit
CCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGG3140.10366731376200097No Hit
CCTATTCTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG3140.10366731376200097No Hit
GGCCGACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATC3120.10300701240045958No Hit
TCTCAGAAGATCAAGGTCGGTCGGCGGTGCAACCCTCAAGGGGATCCCGCC3120.10300701240045958No Hit
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC3090.10201656035814746Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTGCACATGGGTTAGTCGATCCTAAGAGACGGGGGAAGCCCGTCTGATA3040.10036580695429395No Hit
ATGATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTA3040.10036580695429395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)