FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006215715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302892
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC27840.9191394952656392Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT21880.7223696895262999Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG12380.40872654279413123Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT10990.3628355981670034Clontech SMART CDS Primer II A (96% over 26bp)
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT10260.33873459847074205No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC10100.3334521875784108No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG8890.29350395520515565No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG8270.273034612997372No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA7920.2614793391703974No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA7830.25850798304346106No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC7420.24497180513186217No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT7200.2377084901549067No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA6950.2294547231356391No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG6940.2291245724548684No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT6800.22450246292407852No Hit
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC6450.21294718909710392No Hit
GTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGG6300.20799492888554338No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT6100.20139191527012928No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA6070.20040146322781718No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA6010.19842055914319295No Hit
GCTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT5850.19313814825086167No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTACTATGTCTGG5800.19148739484700816No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA5750.18983664144315465No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC5730.18917634008161324No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAA5630.18587483327390622No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC5260.17365925808539018No Hit
GACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAAT5250.17332910740461946No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGC5140.16969744991614172No Hit
GAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCA5100.16837684719305893No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA4950.16342458698149837No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT4790.1581421760891671No Hit
GGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC4730.15616127200454286No Hit
GCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCG4720.15583112132377216No Hit
GTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTT4680.15451051860068934No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA4630.15285976519683583Clontech Universal Primer Mix Long (96% over 25bp)
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC4610.15219946383529442No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGG4560.1505487104314409No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGT4540.1498884090698995No Hit
ACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTC4540.1498884090698995No Hit
GGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG4540.1498884090698995No Hit
GCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCG4520.1492281077083581No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA4450.14691705294296317No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAG4410.14559645021988035No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACCTGT4370.14427584749679756No Hit
CCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGA4320.14262509409294402No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCT4310.1422949434121733No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTG4300.1419647927314026No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTAT4250.1403140393275491No Hit
GCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCACT4190.13833313524292487No Hit
CCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCA4170.13767283388138346No Hit
CTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAAGCGGGGCTCTGC4160.13734268320061277No Hit
GCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCT4120.13602208047752995No Hit
GATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC4120.13602208047752995No Hit
GCTGATGACTCGCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCA4050.133711025712135No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4030.13305072435059362Clontech SMART CDS Primer II A (96% over 26bp)
CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTG4000.1320602723082815No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAACTTACCA3970.1310698202659694No Hit
GCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATA3960.1307396695851987No Hit
GATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGA3910.12908891618134516No Hit
CGCTTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACG3890.12842861481980375No Hit
TCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTA3890.12842861481980375No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGCTGT3820.12611756005440886No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCT3780.12479695733132602No Hit
CTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCT3750.12380650528901391No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCCTGTCTCTTATA3720.1228160532467018Clontech SMART CDS Primer II A (96% over 26bp)
CCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAA3700.1221557518851604No Hit
ACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGG3690.12182560120438969No Hit
CCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTT3630.11984469711976546No Hit
ACTCTGGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACT3610.11918439575822404No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGT3590.11852409439668266No Hit
GCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTT3570.11786379303514125No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG3560.11753364235437054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTT3540.11687334099282913No Hit
GGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGT3540.11687334099282913No Hit
GCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTA3520.11621303963128772No Hit
TCTCAGAAGATCAAGGTCGGTCGGCGGTGCAACCCTCAAGGGGATCCCGCC3500.11555273826974631No Hit
GGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGT3460.1142321355466635No Hit
CATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAA3460.1142321355466635No Hit
CCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAG3440.1135718341851221No Hit
GACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTG3440.1135718341851221No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCT3380.11159093010049788No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGTATGCTCTCACTC3360.11093062873895647No Hit
TGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGA3350.11060047805818575No Hit
CGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCA3350.11060047805818575No Hit
GAGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGAT3280.10828942329279083No Hit
ACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGA3260.10762912193124942No Hit
GCCGGGGGACGGACTGGGAACGATCGCTTCGGCGGTCTTCCCCGGGCGTCG3230.10663866988893732No Hit
GGGCAGAGCCCCGCTTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGT3140.10366731376200097No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTTC3100.10234671103891817No Hit
CCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGAATTTC3070.10135625899660605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)