FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159388
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC5510.3456972921424448No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT5230.32813009762340956No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA4120.25848871935151957No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC4120.25848871935151957No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC4030.2528421211132582No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG3870.24280372424523802No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA3730.2340201269857204No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA3680.23088312796446406No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT3660.22962832835596156No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA3660.22962832835596156No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT3530.22147213090069517No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG3500.21958993148794137No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG3280.20578713579441363No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA3170.19888573794764977No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAA3130.1963761387306447No Hit
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC3120.19574873892639344No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC3120.19574873892639344No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA3110.1951213391221422No Hit
GCTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT3000.1882199412753783No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTAT2900.1819459432328657No Hit
GTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGG2890.18131854342861445No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT2820.17692674479885562No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGG2780.1744171455818506No Hit
CTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAAGCGGGGCTCTGC2730.1712801465605943No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTACTATGTCTGG2650.16626094812658418No Hit
GGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC2610.16375134890957913No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAG2610.16375134890957913No Hit
CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTG2510.15747735086706652No Hit
CCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGA2440.15308555223730771No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTG2430.15245815243305644No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCT2330.14618415439054383No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCT2280.14304715536928753No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT2220.13928275654377995No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA2150.13489095791402114No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC2130.13363615830551862No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGC2130.13363615830551862No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA2110.1323813586970161No Hit
GACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAAT2080.1304991592842623No Hit
GCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTT2030.127362160263006No Hit
GGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG2010.12610736065450348No Hit
GCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCG1970.12359776143749844No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGT1970.12359776143749844No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCT1970.12359776143749844No Hit
GATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC1960.12297036163324718No Hit
CCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCA1960.12297036163324718No Hit
GGCCTAAGCGGCCATAGTCCCTCTAAGAAGCTGGCCGCGGAGGGTCACCTC1940.12171556202474465No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAACTTACCA1910.11983336261199087No Hit
GTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGA1910.11983336261199087No Hit
CCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAG1900.1192059628077396No Hit
GGGCAGAGCCCCGCTTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGT1890.11857856300348833No Hit
CGCTTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACG1890.11857856300348833No Hit
GCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCACT1860.11669636359073457No Hit
GACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTG1850.11606896378648329No Hit
GCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATA1840.11544156398223203No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGTATGCTCTCACTC1820.11418676437372952No Hit
TGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGA1820.11418676437372952No Hit
GTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTT1810.11355936456947827No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAG1790.11230456496097573No Hit
GACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGG1780.11167716515672448No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACCTGT1770.11104976535247321No Hit
GAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCA1760.11042236554822196No Hit
GCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTT1740.10916756593971942No Hit
CCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTG1740.10916756593971942No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGCTGT1720.10791276633121691No Hit
GTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGAGAGGTGTAGGA1710.10728536652696563No Hit
GCCGAGGCGAGCCTTGGGTCCAAAAAGAGGGGCAGAGCCCCGCTTCCGATT1710.10728536652696563No Hit
TCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTA1700.10665796672271438No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGA1700.10665796672271438No Hit
ACTTGGAGCTCTCGATTCCTTGGCGCGGCTCAACGAAGCAGCCGCGCCGTC1700.10665796672271438No Hit
CGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCA1690.10603056691846312No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTTC1690.10603056691846312No Hit
GCTGATGACTCGCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCA1690.10603056691846312No Hit
ACTATGTCTGGACCTGGTAAGTTTCCCCGTGTTGGGTCAAATTAAGCCGCA1670.1047757673099606No Hit
AGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCC1660.10414836750570933No Hit
GATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGA1660.10414836750570933No Hit
GCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCG1640.10289356789720681No Hit
CCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAA1640.10289356789720681No Hit
CCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGA1640.10289356789720681No Hit
GGCCGACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATC1620.1016387682887043No Hit
ACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGA1620.1016387682887043No Hit
GCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTA1620.1016387682887043No Hit
CCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACAGAATAGG1610.10101136848445302No Hit
CCGCGGCCAGCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTG1600.10038396868020177No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)