FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159388
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT5480.3438150927296911No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC5040.3162095013426356No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC4300.2697819158280423No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA4190.26288051798127837No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG3980.2497051220920019No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG3720.23339272718146914No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT3680.23088312796446406No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA3590.22523652972620273No Hit
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC3560.22335433031344895No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG3480.21833513187943884No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT3350.21017893442417246No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC3300.20704193540291616No Hit
GTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGG3220.20202273696890605No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA3170.19888573794764977No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA3050.1913569402966346No Hit
GCTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT2940.18445554244987075No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAA2870.18006374382011195No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC2860.17943634401586067No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA2760.17316234597334806No Hit
CTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAAGCGGGGCTCTGC2710.17002534695209176No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTACTATGTCTGG2710.17002534695209176No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA2640.16563354832233293No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT2620.1643787487138304No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGG2550.15998695008407157No Hit
GGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC2500.15684995106281527No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTAT2450.15371295204155896No Hit
CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTG2440.15308555223730771No Hit
GACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAAT2400.15057595302030266No Hit
CCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGA2310.1449293547820413No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAG2270.14241975556503625No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCT2230.13991015634803122No Hit
GGGCAGAGCCCCGCTTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGT2170.13614575752252364No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAACTTACCA2150.13489095791402114No Hit
GGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG2140.13426355810976986No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTG2130.13363615830551862No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA2090.13112655908851356No Hit
GCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTT2080.1304991592842623No Hit
GCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCACT2060.12924435967575978No Hit
GCTGATGACTCGCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCA2050.12861695987150853No Hit
GGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGGAATCCGACTGT1970.12359776143749844No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC1960.12297036163324718No Hit
GCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTA1960.12297036163324718No Hit
GCCGGGGGACGGACTGGGAACGATCGCTTCGGCGGTCTTCCCCGGGCGTCG1960.12297036163324718No Hit
GCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCG1950.1223429618289959No Hit
CCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCA1910.11983336261199087No Hit
CGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCA1910.11983336261199087No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCT1890.11857856300348833No Hit
TCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTA1880.11795116319923708No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGT1870.11732376339498582No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT1870.11732376339498582No Hit
GCCGAGGCGAGCCTTGGGTCCAAAAAGAGGGGCAGAGCCCCGCTTCCGATT1850.11606896378648329No Hit
CCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTG1850.11606896378648329No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA1840.11544156398223203No Hit
GTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGAGAGGTGTAGGA1830.11481416417798078No Hit
GTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTT1830.11481416417798078No Hit
GCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTT1820.11418676437372952No Hit
CCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTT1820.11418676437372952No Hit
GCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATA1820.11418676437372952No Hit
GTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGA1820.11418676437372952No Hit
CCTATTCTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGG1820.11418676437372952No Hit
GCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCG1810.11355936456947827No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACCTGT1790.11230456496097573No Hit
GATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGA1760.11042236554822196No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGTATGCTCTCACTC1760.11042236554822196No Hit
GACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTG1750.10979496574397068No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGC1740.10916756593971942No Hit
CGCTTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACG1740.10916756593971942No Hit
GGCCTAAGCGGCCATAGTCCCTCTAAGAAGCTGGCCGCGGAGGGTCACCTC1740.10916756593971942No Hit
GGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGT1730.10854016613546817No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCT1700.10665796672271438No Hit
ACTCTGGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACT1680.10540316711421187No Hit
TGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGA1680.10540316711421187No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGCTGT1640.10289356789720681No Hit
CCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGA1630.10226616809295556No Hit
ACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGG1620.1016387682887043No Hit
GAGTACATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCA1620.1016387682887043No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)