FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292635
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC7650.2614178071659234No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT6280.21460180771267962No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA5970.20400840637654413No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC5690.1944401729116476No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG5440.18589710731799No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA5030.17188647974439147No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA4970.16983614400191363No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA4900.1674440856356895No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC4870.1664189177644506No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT4680.15992618791327082No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA4660.15924274266577818No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAA4640.15855929741828556No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC4440.15172484494335947No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA4350.14864934132964272No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA4280.14625728296341858No Hit
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT4200.14352350197344815No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG4160.14215661147846292No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG4120.14078972098347772No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT4090.1397645531122388No Hit
GCTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT4000.13668904949852206No Hit
GCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCG3910.1336135458848053No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGC3600.12302014454866986No Hit
CCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGA3600.12302014454866986No Hit
GGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC3580.12233669930117723No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC3440.11755258256872896No Hit
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC3380.11550224682625114No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGG3300.11276846583628068No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTACTATGTCTGG3300.11276846583628068No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC3240.11071813009380287Clontech SMART CDS Primer II A (96% over 26bp)
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGT3080.10525056811386196No Hit
GTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGG3010.10285850974763784No Hit
CCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGG3000.10251678712389155No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTG2990.10217506450014523No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTAT2990.10217506450014523No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA2980.10183334187639893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)