FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285119
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCC11270.39527355244652235No Hit
CCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCACGGGGGTCCCA10150.3559917087251288No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT9050.3174113264987602No Hit
CCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGG8870.3110981730435362No Hit
AGGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGAC7740.27146559857463026No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA7460.2616451376442818No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGAGGC7400.2595407531592072No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGGCCGA7290.25568271493657035No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA7140.2504217537238837No Hit
CTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAA7070.24796663849129663No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC6940.24340713877363485No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCT6780.23779544681343578No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT6700.23498960083333625No Hit
GCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTGAGGCAATAA6440.22587060139801274No Hit
CCTAAGAGACGGGGGAAGCCCGTCTGATAGCGTGCTAAGCGCGAGCTTCGA6420.22516913990298787No Hit
GACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCA6200.21745306345771412No Hit
GGCCTGTCGGCCAAGGCTATAAACTCGTTGAATACATCAGTGTAGCGCGCG6130.21499794822512705No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC6120.21464721747761462Clontech SMART CDS Primer II A (96% over 26bp)
GAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTT6000.21043844850746532No Hit
GCTAACTAGCTATGCGGAGGTGACCCTCCGCGGCCAGCTTCTTAGAGGGAC5950.2086847947699031No Hit
GCTTAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT5920.20763260252736576No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTC5870.20587894878980353No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA5780.20272237206219157No Hit
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG5770.2023716413146791No Hit
GCTCTCGCCGCGGGAGCAGCTCGAGCAGTCCGCCGACAGCCGACGGGTTCG5740.20131944907214183No Hit
GGTTACGGATCCATTTTGCCGACTTCCCTTGCCTACATTGTTCCATCGACC5140.18027560422139527No Hit
CCGCACGTCGCGTGGTGTCCGGTGCGCCCCCGGCGGCCCTTGAAAATCCGG4990.17501464300870864No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT4900.17185806628109668Clontech Universal Primer Mix Long (96% over 25bp)
AGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGGGCCTCCACCAGAGT4860.17045514329104688No Hit
GTCGGCTGTCGGCGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGC4490.15747810563308653No Hit
GTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGG4480.15712737488557407No Hit
TGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGA4430.1553737211480119No Hit
GCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCGATTCCCTTTCG4380.15362006741044967No Hit
CCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTG4320.151515682925375No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTTC4280.15011275993532525No Hit
GCTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA4280.15011275993532525No Hit
GTCTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCAGAATCAG4190.14695618320771328No Hit
GTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCT4150.1455532602176635No Hit
GTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT4140.14520252947015105No Hit
CCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGG4110.14415033722761372No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGGGCTGT4070.14274741423756396No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTAT4020.14099376050000176No Hit
CTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAGCTAGTTAGCAGGCTG4020.14099376050000176No Hit
GTCTCTTAGGATCGACTAACCCATGTGCAAGTGCCGTTCACATGGAACCTT3990.13994156825746443No Hit
GCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGATTA3960.1388893760149271No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTG3940.13818791451990223No Hit
GCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATA3910.1371357222773649No Hit
ACTCTGGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACT3850.13503133779229023No Hit
GCGCTTACTAGGAATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC3850.13503133779229023No Hit
GATGAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTGGGGCGCGAGCCCGG3830.13432987629726534No Hit
CCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACCTGT3820.1339791455497529No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCT3810.13362841480224047No Hit
CCGCATAGCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCA3790.13292695330721557No Hit
GACAAATCACTCCACCAACTAAGAACGGCCATGCACCACCACCCATAGAAT3710.13012110732711604No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTACTATGTCTGG3710.13012110732711604No Hit
ACAAAGGGTGTTGGTCGATTAAGACAGCAGGACGGTGGTCATGGAAGTCGA3680.12906891508457874No Hit
TCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTA3650.12801672284204138No Hit
GTTCTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGA3630.1273152613470165No Hit
GCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTTTGCCGACTT3570.12521087686194185No Hit
CCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTTACACACTCCTTAG3450.12100210789179255No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGTATGCTCTCACTC3440.1206513771442801No Hit
CTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAAGCGGGGCTCTGC3430.12030064639676767No Hit
GCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATTCCTGAACCGGGAC3430.12030064639676767No Hit
GCTGACGGCAACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAG3350.11749480041666813No Hit
CATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGATGGAACAATGTAGGCAA3330.11679333892164324No Hit
CTTAGTTGGTGGAGTGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCT3290.11539041593159347No Hit
GCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGTT3270.11468895443656858No Hit
GTTATTGCCTCAAACTTCCTTGGCCTAAGCGGCCATAGTCCCTCTAAGAAG3270.11468895443656858No Hit
GATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCATGCGCGTCAC3230.11328603144651882No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAACTTACCA3230.11328603144651882No Hit
CCGGCTGAGCCGTTTCCAGGGTGGGCAGGCTGTTAAACAGAAAAGATAACT3210.11258456995149393No Hit
ACTTGGAGCTCTCGATTCCTTGGCGCGGCTCAACGAAGCAGCCGCGCCGTC3180.11153237770895662No Hit
TTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCG3130.1097787239713944No Hit
GAAGAGGGGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGAT3100.10872653172885706No Hit
CGCTTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACG3080.10802507023383219No Hit
CCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGACGGTTCGA3080.10802507023383219No Hit
CCAAGGAGTCTGACATGTGTGCGAGTCAACGGGCCAGTAAACCCGTAAGGC3070.10767433948631974No Hit
CCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCCGAGGTTACGGATCCATTT3060.10732360873880731No Hit
TCTCAGAAGATCAAGGTCGGTCGGCGGTGCAACCCTCAAGGGGATCCCGCC3050.10697287799129487No Hit
GGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCT3050.10697287799129487No Hit
CCGCGGCCAGCTTCTTAGAGGGACTATGGCCGCTTAGGCCAAGGAAGTTTG3010.1055699550012451No Hit
CGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGATTCCCACTGTC2980.10451776275870776No Hit
GATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGACCCGAAAGA2970.10416703201119532No Hit
GCAAAATGGATCCGTAACCTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCA2960.10381630126368288No Hit
GGTCTTCCCCGGGCGTCGAACAGTCGACTCAGAACTGGTACGGACAAGGGG2950.10346557051617045No Hit
GCCGGGGGACGGACTGGGAACGATCGCTTCGGCGGTCTTCCCCGGGCGTCG2950.10346557051617045No Hit
GCCGTTTACCCGCGCTTGGTTGAATTTCTTCACTTTGACATTCAGAGCACT2930.10276410902114556No Hit
GACAGGCCCGGGTAATCTTTGAAATTTCATCGTGATGGGGATAGATCATTG2920.10241337827363313No Hit
CCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGA2920.10241337827363313No Hit
ACTATGTCTGGACCTGGTAAGTTTCCCCGTGTTGGGTCAAATTAAGCCGCA2910.10206264752612067No Hit
CCTACTCATCGGGGCCTGGAACTTGCCCCGACGGCCGGGTATAGGTCGCGC2890.10136118603109578No Hit
GGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGGGGCGCA2870.10065972453607092No Hit
TCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCACG2860.10030899378855847No Hit
ACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTC2860.10030899378855847No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)