FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262371
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTAT18780.7157803263317973No Hit
GGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTT15390.5865739734955464No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTT14570.5553205194171612No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAA13490.5141574335578246No Hit
ACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGAC12800.4878587953699151No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGG11380.4337369602585651No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAA11100.42306504910984827No Hit
AGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCG9650.3677997949468501No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG8650.3296858265585755No Hit
CCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCC8620.3285424075069272No Hit
CCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTC8280.3155836582549138No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTC7960.30338718837066597No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC7950.3030060486867832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7520.2866170422798251No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7390.2816622263893494No Hit
GCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAG7310.27861310891828744No Hit
GCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATT7030.2679411977695706No Hit
CCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAA6950.2648920802985086No Hit
GGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATC6810.2595561247241502No Hit
CCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATGGGCATGA6160.23478204527177166No Hit
GATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTG6140.23401976590400617No Hit
ACTACGGCGGACTAATCTTCAACTCCTACATACTTCCCCCATTATTCCTA5880.22411013412305475No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACT5800.2210610166519928No Hit
CCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAGCTCATGAGTG5750.21915531823257905No Hit
CTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCC5610.21381936265822063No Hit
ACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCTACATACTTCCC5290.20162289277397272No Hit
GAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAG5260.2004794737223245No Hit
CCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCA5170.19704921656737978No Hit
GCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGACCGGG5080.19361895941243507No Hit
GCCATCATCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACAT5030.19171326099302133No Hit
ACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTAT4990.19018870225749035No Hit
GAGTCATGGAGCGTGCCAGTCCTGTCTCTTATACACATCTCCGAGCCCAC4790.18256590857983543No Hit
GGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGA4700.1791356514248907No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTA4410.16808260059229108No Hit
GCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGATGGGCATG4310.1642712037534636No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT4180.1593163878629879No Hit
GGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTATCATAG4150.15817296881133966No Hit
ACACGACCGGGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAA4070.1551238513402777No Hit
GCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAAGCCCCCA3800.14483307987544355No Hit
CCATTGGTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGT3530.1345423084106094No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA3530.1345423084106094No Hit
TATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTT3480.13263660999119567No Hit
CCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC3310.126157235365189No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGGGC3300.12577609568130624No Hit
GCTCTAGAGGGGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTT3270.124632676629658No Hit
GAGTACATGGAGCGTGCCAGTCCTGTCTCTTATACACATCTCCGAGCCCA3240.12348925757800977No Hit
GCATGAAACTGTGGTTTGCTCCACAGATTTCAGAGCATTGACCGTAGTAT3210.12234583852636152No Hit
ACCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTC3200.12196469884247878No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA3200.12196469884247878No Hit
CATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCA3180.12120241947471329No Hit
GATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAG3070.11700988295200308No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGA3020.11510418453258935No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT3010.1147230448487066No Hit
AAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGGACGA3000.11434190516482386No Hit
GCAGGTCGCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAG2980.11357962579705835No Hit
GTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATTTGATGGTAAG2890.11014936864211365No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGC2870.10938708927434816No Hit
GGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCT2800.10671911148716894No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT2790.10633797180328618No Hit
CCAATGGTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCA2630.10023973686116225No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)