FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256653
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8060.3140426957799051No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTAT7930.30897749100926153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6950.27079363966133263No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTT5870.22871347695137015No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAA5630.2193623296824896No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG5550.21624528059286272No Hit
ACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGAC5500.21429712491184594No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGG5250.20455634650676205No Hit
GGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTT5140.2002704040085251No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAA4780.1862436831052043No Hit
GTTCTAAGTTGGTCGTTAAGCGCACGCCGGACGGCCGAAGCCTGCCAAGG4490.17494438015530697No Hit
AGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCG4260.16598286402262977No Hit
GCCGTAGTCGGTGTACTCGTAGGTTCAGTACCATTGGTGGCCAATTGATT3780.14728056948486867No Hit
GAGTCATGGAGCGTGCCAGTCCTGTCTCTTATACACATCTCCGAGCCCAC3780.14728056948486867No Hit
CCCATCCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCC3530.13753979107978478No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTC3270.1274093815384975No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTAC3240.12624048812988745No Hit
CCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTC3210.12507159472127735No Hit
CTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTCCCATCCC2800.10909671813693976No Hit
GCGTAGGGATGGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAG2750.10714856245592297No Hit
GAGTACATGGAGCGTGCCAGTCCTGTCTCTTATACACATCTCCGAGCCCA2730.10636930018351626No Hit
CCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAA2660.10364188223009277No Hit
GATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTG2650.10325225109388941No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)