FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006215910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006215910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17567498
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGCTAACTGGGTTCTGTTTTGCGTGA1039360.5916380351943117No Hit
CGTCAAAAGTAGGAAGCAGGACCATCAC827360.4709606342348808No Hit
GTCATTTGTGTGTGGACGCAGCCCCTTA742450.4226270582185352No Hit
TGTCCCAAGTAGATCAAGCCTTTACAAA718340.4089028500245169No Hit
CTCTCAGTCGTCGATATAGCGCACCGAG706230.40200943811122103No Hit
TCCTGCAAGACTGGGTCCTCGTCAAAGT694530.3953494117375166No Hit
GGGTATTGTGTCATCAGTAGGATCGAAA636200.3621460494829713No Hit
CGTAAGTGTCGGCACTACCTCACTCAGG632890.3602618881755387No Hit
TGGAACTGTTCCGTTCCCACCGTCTCTT612190.3484787645912924No Hit
TTTCATGCAAGAGGTCAATCTTACTCCT599700.34136904412910707No Hit
ATTCCCGGTCATATGCATCACATGTGCA584430.33267685586188767No Hit
CCCTCAAGTGAGTTGGATTTATATTAGG578140.3290963801447423No Hit
GGATCTACAGCTGTATGCTCCTTCGATC554000.3153550949600222No Hit
GTTACAGGTGTGTCCGGATTAATCGAGT501590.28552159220396667No Hit
CCGTGAGAGCGCATCCTGACATACTCCA477510.2718144610006502No Hit
ATCTCTAGTCACTTCCTATCCAATGGAC475720.27079553388877575No Hit
TTATTGCCACCCAACGCTGGTACCAGCC432630.246267282910748No Hit
CAACCTCGTTGACTGTGGGCCCGCTACT431890.2458460504734368No Hit
TCTAACTAGATGATTGACTATCCTTAGT423930.24131495560722419No Hit
TCTTGCGAGCGCCGTTTTTCTTAGTTTT415020.23624308936878777No Hit
TCACGCTAGTAACCTCCACTCTTCGTCC414480.23593570353615526No Hit
TCTTAGTCACCCTAGGCGGTTTCTGCTC412430.23476877583819847No Hit
TTGATGGTCTTCTGGCCATCCTCATGGG390990.22256441981664093No Hit
CAGCACGAGTAGCTCTATACAATCGGTA388240.2209990290023087No Hit
GAACACTTCAATCCGAGCGTTTACGCTA380170.2164053185035228No Hit
TGAGGTTCAACTGCCGGAAGACGACGTA371290.21135052925578815No Hit
CGTAGTAGTCCAACGCATTCTAATATCC363790.2070812815803366No Hit
TAGGAGGCACTGGATTGAATCGTAGATT363670.20701297361752938No Hit
AATCGACCAGAAGCGTAATAGGCTGACC362730.20647789457553944No Hit
CAGCAGCCAGTGGTGATTTAAATTCCCC362050.20609081611963181No Hit
CGTCCATTCACCTCACACATTGGCGTAT352450.2006261790950538No Hit
ACTTCGCTCGTTCAGACTGCGTTTTATC349070.19870217147598368No Hit
ACCGTTCAGTTCCATGTATCCTCAAGTG346640.19731893522913735No Hit
GTTGTAGAGTTGCCTACTAATAATCAGT331370.18862674696191797No Hit
TTAGGGTAGGTAAGTTATTGTAATTCTT317100.18050379171809214No Hit
CACTAAGGTAGCACGATGCATATAACAC309750.1763199289961496No Hit
GAGACCCGTTCTGAGTCGGAATAGGAGC309660.17626869802404418No Hit
CACTGAACACACTTAGCCCTCGCGTGGG309460.17615485141936546No Hit
AAGAACAAGAATTCAGACCATGTTCGAG297970.16961436398057367No Hit
GCTTCACGTCTACATGCCTATTTATAAC297740.16948344038519317No Hit
CTGTGCTGTCAAGACTAGTCTTCCTTTT288630.16429772754207797No Hit
CATAAGCTCTGGTCAAATTCTTGTCGTT285710.16263556711376886No Hit
GGGACTCCATGGCCCATCCAATTCCAAG285500.1625160281788562No Hit
AAGCATCGTGATACTCCCAGGCTTAATG284810.16212325739271466No Hit
GGGTTTACATACCGTACCTGTTATTATT283330.16128079251809224No Hit
AGAAGCGCACCCAAGCCACACCTTGTAC283160.16118402290411532No Hit
GCGAGAATCAATGCACTTAATTAAACCT281840.16043263531323584No Hit
TTATTGCGTTGCGGCTATCACCATCTTT279870.15931124625715057No Hit
CGTAATGAGCCGAATGGAGAATAATACA279270.15896970644311445No Hit
GCTGAATTCTTTCCAAATCTCCACCATA279000.1588160135267982No Hit
GAACGTTAGCCACTCGCGTTGATAATAC277300.15784831738702915No Hit
TCAGGGCCAAGTCCATATTTGAGCTCCC277260.15782554806609342No Hit
CAGCAGCTCCGGTAGCCTCTCTAACATA273870.1558958481167893No Hit
CCGTAGGCAGGATCTTATTAAACGTTAT273600.15574215520047305No Hit
CGTTGGGTCCAATCTTGCTTACACAGCA266450.15167213908320923No Hit
ACGGAAGTCATCACTTCATGAAATTTAA266220.1515412154878287No Hit
CTGAATGAGCTACAAACGCAACGTCGGT264810.15073859692484382No Hit
CCTCCTCCACCTGAATAACCACGATTGT261270.14872351202203068No Hit
AGCCACGTCTTTCTAGAGTTAGACTATT260150.14808597103582993No Hit
TGAACGTTCTTTCGATAATACCATTTCA260110.14806320171489418No Hit
AGTGATCTCTTCTAACCTATTGAGTGAC251910.14339549092306714No Hit
TCCGGGATCTCGGTAAGTACATAAGGAA245300.13963286063843583No Hit
GCAGGCTTCCAACTAGAGGTTGCCCCGG243850.1388074727545152No Hit
AGTGATCGTACCGGAAACCCAATCTAAC242010.13776008399147105No Hit
TTGCATTCACACTTAGACTACAACCCTC234250.13334283572993716No Hit
TTGTTTGTCGATTGACAATATCTTACGG233640.13299560358566712No Hit
TTTCACAAGGTGAGCTCATCATTCCCCT231630.13185144520864608No Hit
ACGCACGAGGTGCGATCGGTCTCCAGTT231530.13179452190630675No Hit
TATTGGGCACTCCCTAACGCAACGGGAA230960.13147005908297243No Hit
CTCCTCCCATACTGACTTCATGTTCTGA220670.12561265127225288No Hit
TGAGCGCTCAGCCCAGATCCCTTGGAGG219470.12492957164418064No Hit
GCATGATTCTACTGCCATAGATCTCCTC216250.12309664130885344No Hit
TCGCAGGCAGAGTTCTTTGTGCCAAGAT215220.12251033129475808No Hit
GACCGCCTGAGTGTCTCTCTCTTTCCGT214290.12198094458300207No Hit
ATTTACCAGACACACGAATCCTGCTGAC207950.11837200721468703No Hit
TTTACCACAACAACAATCTCGGGATGCT206040.11728477214000538No Hit
TGAGCATTCTCACCCATAGCCGGTCATT204650.11649353823748834No Hit
TAGGAGGTCTGTAACGTCATCACTGTCA202920.11550876510701752No Hit
CTGCCATGTAGACAGCACCGATTTACCT201930.11494522441385792No Hit
GAGTCATTCTTAGCTTCGCCTGTGCGGT200460.11410845186946941No Hit
TGCATGAAGCATGATAGCACCAATACTA199300.11344814156233288No Hit
TTGACCCTCGCAACATGCAGGATGCTCT198970.11326029466461303No Hit
GTTACGATCCTAGCTCCCCTGGCCTATA194380.11064751508723666No Hit
AAGTCGTCACGGCACTTTACATCCTGGA193750.11028889828249874No Hit
ATTACCTAGGCTGAACTGCTGTAACGTC189470.10785258094237439No Hit
GCTCAAAAGTGGGAAATGATCCCCGCAT188060.10704996237938949No Hit
TGAATGCAGGTGCTAGGTGTTAGTTGGG186380.10609365090008832No Hit
CTGGACGTCACCCTCACCTCCTATCCGT184940.10527395534640163No Hit
GACCCAGAGGATAATCACTACCCCGTAT178400.10155117137340787No Hit
CTAAGTGTCTTCTGGCAGATTGCTCGAA176720.10059485989410671No Hit
AACGTCACATAACAGATTGGTCCGGCAT176390.10040701299638685No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)