FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACACTGAGCGAT9943.80530973451327No Hit
GGTTTCCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA3214.15929203539823No Hit
ATGACCGGCCCAGCAGTCTCTGCGTTGATACCACTGCTTTTTAAGGAAGCA2912.831858407079647Clontech Universal Primer Mix Long (95% over 23bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG156.637168141592921No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG125.3097345132743365No Hit
ATGACCGGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACACTG31.3274336283185841No Hit
GGTTTCCAAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTGA20.8849557522123894No Hit
ATGACCGGCCCATCAATCATCACAGGGTTGGACCTGTCTCTTATACACATC10.4424778761061947No Hit
GGTTTCCCAGTCCACTATACATCTCCGAGCCCACGAGACACTGAGCGATCT10.4424778761061947No Hit
GGTTTCCCAGTCCACTATACACATCTCCGAGCACACGAGACACTGAGCGAT10.4424778761061947No Hit
GGTTTCCCAGTCCACTATACAATCTCCGAGCCCACGAGACACTGAGCGATC10.4424778761061947No Hit
GGTTTCCCAGTCGACTATACACATCTACGAGCCCACGAGACACTGAGCGAT10.4424778761061947No Hit
GGTTTCCCAGTCCACAATACACATCTCCGAGCCCACGAGACACTGAGCGAT10.4424778761061947No Hit
GGTTTCCCAGTCCACTATACATCTCCGAGCCCACGAGACACTGAGCGATCG10.4424778761061947No Hit
GGTTTCCCAGTCCACTATACACATATCCGAGCCCACGATACACTGAGCGAT10.4424778761061947No Hit
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCAAGAGACACTGAGCGAT10.4424778761061947No Hit
GGTTTCCCAGGTCCACTATACACATCTCCGAGCCCACGAGACACTGAGCGA10.4424778761061947No Hit
GCTTTCCAGCTTGACATAATCCCAATTGCCTATTTTTGCTTTGATAGCCTA10.4424778761061947No Hit
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACCCTGAGCGAT10.4424778761061947No Hit
GGTTTACCAGTCCACTATACACATCTCCGAGCCCACGAGACACTGAGCGAT10.4424778761061947No Hit
GGTTTCCCAGTCCCTATACACATCTCCGAGCCCACGAGACACTGAGCGATC10.4424778761061947No Hit
GTTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACACTGAGCGAT10.4424778761061947No Hit
GCCATCCAAGGAGCCTGAGCACCTAAGGGAACACAGTTACACTGGTGAATG10.4424778761061947No Hit
GGTTTCCCATCCACTATACACATCTCCGAGCCCACGAGACACTGAGCGATC10.4424778761061947No Hit
GGTTGAAGTGCAGGCACAGGTTGTTGCTGTCTTTGCCCAGGTTCAGCACGA10.4424778761061947No Hit
CTGCCTCCCAGGTTCAAGCAATTCTTGTGCCTCAGCCTCCCAAAGTGCTTG10.4424778761061947No Hit
GGTTTCCCAGTCCACTATACTGACGCTGCCGACGAGCGTGCCAGGCCTGTC10.4424778761061947No Hit
ATGACCTGCCCAGCAGTCTCTTATACACATCTCCGAGCCCACGAGACACTG10.4424778761061947No Hit
TGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACACTGAGCGAT10.4424778761061947No Hit
TTGATATATCTAGGGCCAACTGGATGATTTTGAACATTCCCCATGTACTCC10.4424778761061947No Hit
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGAAACTGAGCGAT10.4424778761061947No Hit
ATGACTGGCCCAGCAGTCTCTGCGTTGATACCACTGCTTTTTAAGGAAGCA10.4424778761061947Clontech Universal Primer Mix Long (95% over 23bp)
ATGACCGGCCCAGCAGTCTCTTATACACATCTCGAGCCCACGAGATCATCA10.4424778761061947No Hit
TCTGATTCATGGTTGTTGATTTTGTCCATACTGAAAGGGAGAAAATTCTTG10.4424778761061947No Hit
GGTTTCCGAGTCCACTATACACATCTCCGAGCCCACGAGACACTGAGCGAT10.4424778761061947No Hit
AGTAACCTGCGAGGCCTGGATAGACCCTCTGCCACATATGTTTACATGATG10.4424778761061947No Hit
ATGACCTTCCCAGCAGTCTCTGCGTTGATACCACTGCTTTTTAAGGAAGCA10.4424778761061947Clontech Universal Primer Mix Long (95% over 23bp)
GTGACCGGCCCAGCAGTCTCTGCGTTGATACCACTGCTTTTTAAGGAAGCA10.4424778761061947Clontech Universal Primer Mix Long (95% over 23bp)
AGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACACTGAGCGAT10.4424778761061947No Hit
GGTTTCCCAGTCCTGTCTCTATACACATCTCCGAGCCCACGAGACACTGAG10.4424778761061947No Hit
GGTTTCATCATATTGGTCAGGCTGGTCTCGAACTCCTGACCTCAGGTAATC10.4424778761061947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)