FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421790
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT61291.4530927712842885No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC50731.2027312169563054No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG48751.1557884255198083No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG43221.024680528224946No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT41320.979634415230328No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT41240.9777377367884492No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT40480.959719291590602No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT37260.8833779843049859No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA37130.8802958818369331No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG36470.8646482846914341No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG35770.848052348324996No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC35380.8388060409208373No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC35050.8309822423480878No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG33940.8046658289670214No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT33750.8001612176675598No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGT32920.7804831788330686No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT31630.7498992389577752No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCGGGTTTGACCGCTCGCC17480.41442423955048724No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTCTGTCTCTTATAC16940.40162166006780625No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTATAC16790.3980653879892838No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATACACA16320.38692240214324664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.34993717252661277No Hit
GAGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAA14050.33310415135493965No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATCTGTCTCTTATACACA13200.3129519429099789No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTA12440.2949334977121316No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCC11880.281656748618981No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAAAAA10250.2430119253657033No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTA9900.23471395718248417No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCT9650.22878683705161335No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATAC9460.22428222575215156No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTCTGTCTCTTATACACATC9280.22001469925792455No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCTT9260.2195405296474549No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9050.21456174873752343No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCT9040.2143246639322886No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTCT8750.20744920458047844No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTT8660.20531544133336496No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCT8430.19986249081296378No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTG8110.19227577704544915No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGCTGTCTCTTATAC8070.1913274378245098No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTTA8040.19061618340880535No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCC7990.18943075938263118No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCT7870.18658574171981318No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCT7820.18540031769363902No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAA7530.17852485834182885No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCTG7470.17710234951041987No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTT7460.17686526470518504No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTC7320.17354607743189737No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCTGTCTCTTATACACATC7240.17164939899001874No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTC6920.16406268522250408No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCTT6730.15955807392304228No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTTA6630.15718722587069395No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6610.1567130562602243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5580.13229332132103652No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA5120.12138742028023423No Hit
TCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATACACAT5030.11925365703312076No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTCTGTCTCTTATACACA4700.11142985846037128No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGCTGTCTC4580.10858484079755329No Hit
TCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAAAAAA4500.10668816235567462No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA4490.1064510775504398No Hit
TCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTG4350.10313189027715214No Hit
GAGTACATGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAA4280.10147229664050832No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGCTGTCTCTTATACACA4270.10123521183527347No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)