FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320489
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT74002.308971602769518No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC57221.7853966906820513No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG52471.6371856756394136No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT51521.607543472630886No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT44551.3900633095051624No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG43351.3526205267575486No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG42821.3360832977106858No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA42761.3342111585733052No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG42701.3323390194359246No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT42091.3133056048725542No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG40141.2524610829076817No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC38601.2044095117149105No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT38281.1944247696488803No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC38091.1884963290471748No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT37801.1794476565498349No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT34581.0789761895104044No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGT34471.0755439344252065No Hit
GAGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAA24500.7644568144304486No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCGGGTTTGACCGCTCGCC24060.7507277940896567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21290.6642973705805816No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATACACA18180.5672581586263491No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTATAC17580.5485367672525422No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTCTGTCTCTTATAC17190.5363678628595677No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAAAAA15020.4686588307242994No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATCTGTCTCTTATACACA14890.46460252925997464No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCC14490.45212160167743665No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTA11780.36756331730574215No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTCTGTCTCTTATACACATC11060.3450976476571739No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10400.3245041171459863No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAA10270.3204478156816615No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTA10050.3135833055112656No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTCT9880.308278911288687No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCTT9620.3001663083600373No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATAC9600.29954226198091044No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCT9290.2898695431044435No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGCTGTCTCTTATAC9130.2848771720714283No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCTG8520.265843757508058No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCT8510.2655317343184946No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCT8430.263035548801987No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTG8420.2627235256124235No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCT8400.26209947923329663No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCTGTCTCTTATACACATC8390.26178745604373316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8300.25897924733766214No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCT8200.25585901544202766No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTT8160.2546109226837739No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTC7850.24493820380730694No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCC7800.24337808785948972No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTC7700.24025785596385524No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTTA7670.23932178639516488No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCTT7580.23651357768909387No Hit
GAGTACATGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAA7560.23588953130996695No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTTA7340.2290250211395711No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTT7330.22871299795000763No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7200.22465669648568282No Hit
TCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAAAAAA6940.21654409355703316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6430.20063091088929727No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAA6000.187213913738069No Hit
TCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATACACAT5750.1794133339989828No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACAAAAAAAAAAAAA5650.17629310210334834No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTCTGTCTCTTATACACA5490.17130073107033314No Hit
TCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTG5290.16506026727906417No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGCTGTCTC5190.1619400353834297No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGCTGTCTCTTATACACA5030.15694766435041452No Hit
AGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAA5020.15663564116085107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG4990.15569957159216072No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGACTGTCTCTTATACACAT4750.14821101504263798No Hit
CAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGTCTC4670.14571482952613038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA4480.13978638892442485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4330.13510604108097313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4170.13011367004795796No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGAAAAAAAAAAAAAAA4090.1276174845314504No Hit
TCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGT3940.12293713668799866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3720.11607262651760278No Hit
CAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGGTTT3710.11576060332803935No Hit
TCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTC3650.11388846419065864No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACAAAAAAAAAA3540.11045620910546071No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGACTG3450.1076480003993897No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA3450.1076480003993897No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGACTGT3320.10359169893506485No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG3270.10203158298724763No Hit
CGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGGTTTGACCGCT3260.10171955979768417No Hit
CCGATTGGTATCATGCTGCACCAATCGAGGATCGGGTTTGACCGCTCGCCT3260.10171955979768417No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)