FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420236
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT38900.9256703376198137No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT31900.7590972691535233No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG31110.7402983085694705No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT28920.6881847342921596No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG28530.6789042347633235No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA27300.6496349670185324No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT26500.6305980449080993No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC26050.6198897762209806No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT25820.6144166611142311No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC25770.613226853482329No Hit
CTTTTCAAAGTTCTTTTCATCTTTCCATCACTGTACTTGTTCGCTATCGGT25250.6008528541105473No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC24550.5841955472639184No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT24420.5811020474209729No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGT24220.5763428168933646No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG23590.5613512407313985No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG23490.5589716254675944No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT23420.5573058947829315No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG22800.5425522801473457No Hit
GAGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAA18820.44784359264794066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17540.4173845172712476No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCGGGTTTGACCGCTCGCC12790.30435279224055056No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACC10990.2615197174920759No Hit
AATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAA10050.2391513340123169No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCTGTCTCTTATACACATC9900.23558191111661067No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAAAAA9290.22106625800740534No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAA8810.20964410474114548No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCC8620.20512283573991758No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8040.1913210672098535No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA7790.18537202905034314No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTCTGTCTCTTATACACATC7530.17918502936445235No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTCTGTCTCTTATAC7270.17299802967856157No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7100.1689526837300945No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCC7050.16776287609819243No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATCTGTCTCTTATACACA7020.1670489915190512No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTATAC6630.15776849199021503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6510.15491295367365004No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCT6280.14943983856690052No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATA6070.14444264651291178No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCT6050.14396672346015096No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTG5920.14087322361720556No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCTG5900.14039730056444474No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATACACA5800.1380176853006406No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCTT5330.1268334935607611No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTT5280.12564368592885902No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTCT5220.12421591677057653No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCT5210.12397795524419612No Hit
GAGTACATGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAA5210.12397795524419612No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTC5140.12231222455953321No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAA5080.12088445540125073No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG5040.11993260929572906No Hit
TCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAAAAAA5030.11969464776934866No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCT4700.11184191739879495No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCT4560.10851045602946917No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTC4540.10803453297670834No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC4510.1073206483975671No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTTA4440.10565491771290418No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTA4440.10565491771290418No Hit
CCCACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCACTTTAC4430.10541695618652377No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC4410.10494103313376293No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC4390.10446511008100211No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTT4340.10327530244910003No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTA4300.10232345634357837No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)