FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445353
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCT53591.2033151230596852No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGT39530.8876105022308147No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTG37320.837986945187301No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATAC36760.8254126501898493No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCAGCATGATACCAA34960.7849952734123268No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTG33520.7526613719903088No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGT33310.7479460113662646No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCT33160.7445778966348043No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGG32410.7277373229775033No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTC31390.7048341428035737No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTC31240.7014660280721136No Hit
CCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCT31200.7005678641437242No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCGGG31140.6992206182511401No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGT30470.6841763724506178No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCG30420.6830536675401311No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGCTGTCT29940.6722757003994584No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCTGT29900.671377536471069No Hit
GAGTACATGGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAA20600.46255442312053585No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCGGGTTTGACCGCTCGCC18180.4082155054529778No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16910.3796988007266146No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATA12500.28067622762168437No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTCTGTCTCTTATACACATC12200.2739399981587639No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCC11270.25305768682371066No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAAAAA11110.24946503111015306No Hit
ACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTATAC10550.23689073611270162No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTCTGTCTCTTATAC10300.23127721156026793No Hit
ATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATACACA10200.22903180173929447No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAA9720.21825383459862174No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC9440.21196668709989605No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATCTGTCTCTTATACACA9380.21061944120731196No Hit
ACCGATTGGTATCATGCTGCACCAATCGAGGATCTGTCTCTTATACACATC9230.20725132647585173No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.1895125888901613No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA8370.18794080201547986No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCT7960.1787346217494886No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC7890.1771628348748072No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC7120.1598731792533114No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA7030.1578523104144353No Hit
ACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACG6980.15672960550394854No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCC6900.15493327764716977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6890.15470873666507243No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCT6820.153136949790391No Hit
AGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG6810.15291240880829365No Hit
GATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTG6800.1526878678261963No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT6770.15201424487990425No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA6740.1513406219336122No Hit
GCACCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTA6600.14819704818424934No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTCT6540.14684980229166528No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGAGGATCCTG6510.14617617934537322No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCCTGTCTC6490.14572709738117853No Hit
GAGTACATGGGGGTATCGACGATTACAAAAAAAAAAAAAAAAAAAAAAAAA6340.1423589826497183No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCTT6270.14078719577503687No Hit
GCGTAGGGATGGTAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGT6160.13831724497196607No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT6150.1380927039898687No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC6140.13786816300777135No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTC6120.13741908104357667No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA6100.13696999907938195No Hit
CTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCC6060.13607183515099258No Hit
GCAATTCCCGGACGTCTAAACCAAACCACTTTCACCGCTACACGACCGGGG6040.1356227531867979No Hit
GGGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCT6020.13517367122260318No Hit
CCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCC5990.13450004827631115No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGTGCCTGTCTCTTA5990.13450004827631115No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCT5830.1309073925627536No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCGCTGTCTCTT5760.12933560568807215No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCCTGTCTCT5720.12843744175968275No Hit
GGCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTT5690.12776381881339074No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5680.12753927783129337No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT5590.12551840899241726No Hit
CCAATCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCCTGTCTCTTATAC5590.12551840899241726No Hit
CGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTT5410.121476671314665No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA5410.121476671314665No Hit
GATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGTGCCTGTCTCTTA5360.12035396640417825No Hit
GGTACTGAGGCGAGCGGTCAAACCCGATCCTCGATTGGCTGTCTCTTATAC5310.11923126149369152No Hit
TCGAGGATCGGGTTTGACCGCTCGCCTCAGTACCGACGAAAAAAAAAAAAA5300.11900672051159418No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT5260.11810855658320478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5220.1172103926548154No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG5170.11608768774432866No Hit
CATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCAT5100.11451590086964723No Hit
GCACAGGGAACGTCACCGATTGGTATCATGCTGCACCAATCCTGTCTCTTA4990.11204595006657639No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACA4900.11002508122770027No Hit
ATCCTCGATTGGTGCAGCATGATACCAATCGGTGACGTTCCCTGCTGTCTC4850.10890237631721354No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC4830.10845329435301884No Hit
CTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATCGCCCTACCATCCCT4800.1077796714067268No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC4710.10575880256785068No Hit
GATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGA4670.10486063863946128No Hit
GGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTACTCCCGATTGAA4580.10283976980058517No Hit
CCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCAAAC4510.10126798292590372No Hit
ATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCT4510.10126798292590372No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)