FastQCFastQC Report
Tue 7 May 2024
EGAF00006216316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29384
Total Bases1.4 Mbp
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAA1020.3471276885379799No Hit
CTGGGCCGGTCATCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGA890.30288592431255107No Hit
GCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATC610.20759597059624282No Hit
GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGT380.1293220800435611No Hit
GTATCAACGCAGAGTACATGGGTCCTGGGTGACAAAGCTAAACACTGTCT370.12591886741083583No Hit
GGTATCAACGCAGAGTACATGGGTCCTGGGTGACAAAGCTAAACACTGTC360.12251565477811055No Hit
ACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGAC350.11911244214538524No Hit
GTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGG340.11570922951265995No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTT330.11230601687993466No Hit
GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT310.10549959161448408No Hit
CATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCA300.10209637898175877No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)