FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18806
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG2201.1698394129533127No Hit
GCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC780.41476124641072No Hit
CTGGGCCGGTCATCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC690.36690417951717536No Hit
GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT500.26587259385302564No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC460.24460278634478358No Hit
GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTG420.22333297883654152No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAG420.22333297883654152No Hit
GTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGGT420.22333297883654152No Hit
CGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGT400.2126980750824205No Hit
AGCATCCGTGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT390.20738062320536No Hit
ACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACT370.19674571945123898No Hit
GCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTGAGGCAATAA370.19674571945123898No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC350.18611081569711793No Hit
GCCTATCGATCCTTTAGACCTTCGGAGTTTGAAGCTAGAGGTGTCAGAAAA340.18079336382005742No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC320.1701584600659364No Hit
CCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAA310.1648410081888759No Hit
GATCTTCTGTGAAGGGTTCGAGTTGGAGCACGCCTGTCGGGACCCGAAAGA300.1595235563118154No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA290.15420610443475485No Hit
GAAATCACATTGCGTTAGCATCCGTGGGGACCATCGCAATGCTTTGTTTTA290.15420610443475485No Hit
GACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATT280.14888865255769435No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC280.14888865255769435No Hit
CCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATG270.14357120068063384No Hit
GCAGAAATCACATTGCGTTAGCATCCGTGGGGACCATCGCAATGCTTTGTT270.14357120068063384No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTGGCCT260.13825374880357333No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC260.13825374880357333No Hit
CAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTT260.13825374880357333No Hit
GCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTC250.13293629692651282No Hit
CATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCAT250.13293629692651282No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCCT250.13293629692651282No Hit
CCGCAGCTAGCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTG250.13293629692651282No Hit
GCTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGC250.13293629692651282No Hit
ACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCG250.13293629692651282No Hit
CTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCC250.13293629692651282No Hit
AGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTGGCC250.13293629692651282No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT250.13293629692651282No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAG250.13293629692651282No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT240.1276188450494523No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATAT240.1276188450494523No Hit
GTTTAGGCCACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT230.12230139317239179No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT230.12230139317239179No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA230.12230139317239179No Hit
CAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTT230.12230139317239179No Hit
GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCC230.12230139317239179No Hit
GAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGTGGGGACCATCGCA220.11698394129533128No Hit
AAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCATTGGC220.11698394129533128No Hit
AATGATTATGAGGGCGTGATCATGAAAGGTGATAAGCTCTTCTATGATCTG220.11698394129533128No Hit
ATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCATG220.11698394129533128No Hit
GGCCTGTCGGCCAAGGTGATATACTCGTTGAATACATCAGTGTAGCGCGCG220.11698394129533128No Hit
CAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGG220.11698394129533128No Hit
GATCTTCTGTGAAGGGTTCGAGTTGGAGCACGCCTGTCGGGCTGTCTCTTA220.11698394129533128No Hit
ACAGTGTTTAGCTTTGTCACCCAGGACCCATGTACTCTGCGTTGATACCTG210.11166648941827076No Hit
CCTCTCGTACTAGGTTGAATTACTATCGCGGCACGGTCATCAGTCTGTCTC210.11166648941827076No Hit
GCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCT210.11166648941827076No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTCTG210.11166648941827076No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCCTCCACCAGAGT210.11166648941827076No Hit
TCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCCACGGATGCTAA210.11166648941827076No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT200.10634903754121025No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA200.10634903754121025No Hit
GCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGCT200.10634903754121025No Hit
AACCCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACT200.10634903754121025No Hit
GATAAGTGGGAGCCTTCGGGCGCAAGTGAAATACCACTACTTTTAACGTTA190.10103158566414974No Hit
ATGCATTTGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCT190.10103158566414974No Hit
TCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAA190.10103158566414974No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA190.10103158566414974No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTCTGACTATTTCTGTATGTCTCC190.10103158566414974No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)