FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18806
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG1991.0581729235350419No Hit
GCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC750.39880889077953846No Hit
ACAGATGACCGGCCCAGCAGTCTCTTATACACATCTGACGCTGCCGACGAA650.3456343720089333No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC500.26587259385302564No Hit
AGCATCCGTGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT470.2499202382218441No Hit
GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT440.23396788259066256No Hit
GTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGGT430.22865043071360205No Hit
ACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACT400.2126980750824205No Hit
GTTTAGGCCACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT370.19674571945123898No Hit
GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTG350.18611081569711793No Hit
CCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAA320.1701584600659364No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTGGCCT320.1701584600659364No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC310.1648410081888759No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATAT310.1648410081888759No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC310.1648410081888759No Hit
GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCC310.1648410081888759No Hit
CGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGT290.15420610443475485No Hit
GAAATCACATTGCGTTAGCATCCGTGGGGACCATCGCAATGCTTTGTTTTA290.15420610443475485No Hit
GCAGAAATCACATTGCGTTAGCATCCGTGGGGACCATCGCAATGCTTTGTT290.15420610443475485No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC270.14357120068063384No Hit
GACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATT270.14357120068063384No Hit
CAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTT270.14357120068063384No Hit
CCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATG260.13825374880357333No Hit
GCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTGAGGCAATAA260.13825374880357333No Hit
CATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCAT250.13293629692651282No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAG250.13293629692651282No Hit
GTTATTGCCTCAAACTTCCGTGGCCTAAACGGCCATAGTCCCTCTAAGAAG240.1276188450494523No Hit
GCCTATCGATCCTTTAGACCTTCGGAGTTTGAAGCTAGAGGTGTCAGAAAA240.1276188450494523No Hit
GGCCTGTCGGCCAAGGTGATATACTCGTTGAATACATCAGTGTAGCGCGCG240.1276188450494523No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCCTCCACCAGAGT240.1276188450494523No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTCTG230.12230139317239179No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGTGG230.12230139317239179No Hit
ACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTA230.12230139317239179No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAG230.12230139317239179No Hit
AATGATTATGAGGGCGTGATCATGAAAGGTGATAAGCTCTTCTATGATCTG220.11698394129533128No Hit
CATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGT220.11698394129533128No Hit
CGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCC220.11698394129533128No Hit
CTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCC220.11698394129533128No Hit
GTATCAACGCAGAGTACATGGGTCCTGGGTGACAAAGCTAAACACTGTCTG220.11698394129533128No Hit
ACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGCTGTCTC220.11698394129533128No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT220.11698394129533128No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT210.11166648941827076No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA210.11166648941827076No Hit
CCCGACAGGCGTGCTCCAACTCGAACCCTTCACAGAAGATCCTGTCTCTTA210.11166648941827076No Hit
GATCTTCTGTGAAGGGTTCGAGTTGGAGCACGCCTGTCGGGACCCGAAAGA210.11166648941827076No Hit
GCTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGC200.10634903754121025No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCT200.10634903754121025No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTCTGACTATTTCTGTATGTCTCC200.10634903754121025No Hit
GATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGT200.10634903754121025No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC200.10634903754121025No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT200.10634903754121025No Hit
CCGTGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCT200.10634903754121025No Hit
GGTCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCT200.10634903754121025No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG200.10634903754121025No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCCT200.10634903754121025No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC190.10103158566414974No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA190.10103158566414974No Hit
CCGCAGCTAGCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTG190.10103158566414974No Hit
ATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCATG190.10103158566414974No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT190.10103158566414974No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT190.10103158566414974No Hit
TCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCCACGGATGCTAA190.10103158566414974No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)