FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16307
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG1590.9750413932666954No Hit
GCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC990.6071012448641688No Hit
GTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGGT700.4292635064696143No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAG560.34341080517569145No Hit
GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTG540.33114613356227385No Hit
GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT510.31274912614214756No Hit
GATCTTCTGTGAAGGGTTCGAGTTGGAGCACGCCTGTCGGGACCCGAAAGA410.2514257680750598No Hit
GCCTATCGATCCTTTAGACCTTCGGAGTTTGAAGCTAGAGGTGTCAGAAAA390.23916109646164224No Hit
CCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAA380.23302876065493344No Hit
CTGGGCCGGTCATCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC380.23302876065493344No Hit
GCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTGAGGCAATAA380.23302876065493344No Hit
AGCATCCGTGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT380.23302876065493344No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA360.2207640890415159No Hit
CAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTT360.2207640890415159No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC350.21463175323480715No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATTAACGAGA350.21463175323480715No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTGGCCT340.20849941742809835No Hit
CATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGT330.20236708162138958No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCCT320.19623474581468078No Hit
ACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACT310.19010241000797204No Hit
GTTTAGGCCACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT310.19010241000797204No Hit
GCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCT300.18397007420126327No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC290.1778377383945545No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA280.17170540258784572No Hit
AAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCATTGGC280.17170540258784572No Hit
CCGCAGCTAGCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTG270.16557306678113692No Hit
ACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCG270.16557306678113692No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA270.16557306678113692No Hit
GACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATT270.16557306678113692No Hit
CCAAGGTGAACAGCCTCTGGCCAATGGAACAATGTAGGCAAGGGAAGTCGG260.15944073097442815No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC260.15944073097442815No Hit
GTTATTGCCTCAAACTTCCGTGGCCTAAACGGCCATAGTCCCTCTAAGAAG250.15330839516771938No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT250.15330839516771938No Hit
GAAATCACATTGCGTTAGCATCCGTGGGGACCATCGCAATGCTTTGTTTTA240.1471760593610106No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATAT240.1471760593610106No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC230.14104372355430184No Hit
CAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTT230.14104372355430184No Hit
TCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAA220.13491138774759306No Hit
CCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGAATTTC220.13491138774759306No Hit
CCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATG220.13491138774759306No Hit
GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCC220.13491138774759306No Hit
GAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGTGGGGACCATCGCA210.1287790519408843No Hit
CTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAGC210.1287790519408843No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT210.1287790519408843No Hit
CCCATAGAATCAAGAAAGAGCTCTCAGTCTGTCAATCCTTGCTATGTCTGG210.1287790519408843No Hit
AATGATTATGAGGGCGTGATCATGAAAGGTGATAAGCTCTTCTATGATCTG200.1226467161341755No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACA200.1226467161341755No Hit
GAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTT200.1226467161341755No Hit
GATAAGTGGGAGCCTTCGGGCGCAAGTGAAATACCACTACTTTTAACGTTA190.11651438032746672No Hit
GCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT190.11651438032746672No Hit
CGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAA190.11651438032746672No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCCTCCACCAGAGT190.11651438032746672No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCGTGCTCCAACTC180.11038204452075795No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC180.11038204452075795No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA180.11038204452075795No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT180.11038204452075795No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTC180.11038204452075795No Hit
GCTACTAGATGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGCT180.11038204452075795No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC180.11038204452075795No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT180.11038204452075795No Hit
CAATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGG180.11038204452075795No Hit
GGTAAAAGCTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAG170.10424970871404918No Hit
GATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGA170.10424970871404918No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT170.10424970871404918No Hit
ACTGATGACCGTGCCGCGATAGTAATTCAACCTAGTACGAGAGGCTGTCTC170.10424970871404918No Hit
CCGTGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCT170.10424970871404918No Hit
CGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCATCTAGTAGCCT170.10424970871404918No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)