FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16307
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG1801.1038204452075797No Hit
GCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC1080.6622922671245477No Hit
GTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGGT670.41086649904948797No Hit
GCCTATCGATCCTTTAGACCTTCGGAGTTTGAAGCTAGAGGTGTCAGAAAA450.27595511130189493No Hit
GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT440.26982277549518613No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAG410.2514257680750598No Hit
ACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACT380.23302876065493344No Hit
GCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCT360.2207640890415159No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC360.2207640890415159No Hit
GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTG360.2207640890415159No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTGGCCT360.2207640890415159No Hit
GATCTTCTGTGAAGGGTTCGAGTTGGAGCACGCCTGTCGGGACCCGAAAGA350.21463175323480715No Hit
CCCACTTATCCTACACCTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCA340.20849941742809835No Hit
AGCATCCGTGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT340.20849941742809835No Hit
ACAGATGACCGGCCCAGCAGTCTCTTATACACATCTGACGCTGCCGACGAA330.20236708162138958No Hit
GGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAA320.19623474581468078No Hit
CCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAA320.19623474581468078No Hit
CCAAGGTGAACAGCCTCTGGCCAATGGAACAATGTAGGCAAGGGAAGTCGG320.19623474581468078No Hit
CCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAA310.19010241000797204No Hit
GTTATTGCCTCAAACTTCCGTGGCCTAAACGGCCATAGTCCCTCTAAGAAG300.18397007420126327No Hit
GCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTGAGGCAATAA300.18397007420126327No Hit
GTTTAGGCCACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT280.17170540258784572No Hit
GAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTGAGAGCTCTTT270.16557306678113692No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCCT260.15944073097442815No Hit
CCGCAGCTAGCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTG260.15944073097442815No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATAT250.15330839516771938No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC240.1471760593610106No Hit
CCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATG240.1471760593610106No Hit
GGCCTATCGATCCTTTAGACCTTCGGAGTTTGAAGCTAGAGGTGTCAGAAA240.1471760593610106No Hit
GGTAAAAGCTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAG230.14104372355430184No Hit
GACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATT230.14104372355430184No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC220.13491138774759306No Hit
CAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTCCCTT220.13491138774759306No Hit
CTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCC210.1287790519408843No Hit
CAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTT210.1287790519408843No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATA210.1287790519408843No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTCTG200.1226467161341755No Hit
CATAGCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGT200.1226467161341755No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC200.1226467161341755No Hit
AGAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTGGCC200.1226467161341755No Hit
CGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAA200.1226467161341755No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT190.11651438032746672No Hit
GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCC190.11651438032746672No Hit
GCTCAGGCATAGTTCACCATCTTTCGGGTCCCGACAGGCGTGCTCCAACTC180.11038204452075795No Hit
GGCCGACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGAT180.11038204452075795No Hit
CCTCTCGTACTAGGTTGAATTACTATCGCGGCACGGTCATCAGTCTGTCTC180.11038204452075795No Hit
ACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAG180.11038204452075795No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC180.11038204452075795No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTCTGACTATTTCTGTATGTCTCC180.11038204452075795No Hit
GCTATTAAAGGTTCGTTTGTTCAACGATTAAAGTCCTACGTGATCTGAGTT180.11038204452075795No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT180.11038204452075795No Hit
ATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGGGTAAC180.11038204452075795No Hit
TCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT170.10424970871404918No Hit
AAGTTACGGATCCGTTTTGCCGACTTCCCTTGCCTACATTGTTCCATTGGC170.10424970871404918No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCG170.10424970871404918No Hit
GGCCTGTCGGCCAAGGTGATATACTCGTTGAATACATCAGTGTAGCGCGCG170.10424970871404918No Hit
GTACGAGAGGAACCGTTGATTCACACAATTGGTCATCGCGCTTGGTTGAAA170.10424970871404918No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATATT170.10424970871404918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)