FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32398
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG1670.5154639175257731No Hit
GCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC950.2932279770356195No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC820.2531020433360084No Hit
CATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCAT750.2314957713439101No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC730.22532255077473917No Hit
GGTAGGTGGTAGTTTGTGTTTAATATTTTTAGTTGGGTGATGAGGAATAGT600.1851966170751281No Hit
GTTTAGGCCACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT600.1851966170751281No Hit
CTGGGCCGGTCATCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC570.17593678622137168No Hit
GCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCT540.1666769553676153No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC520.16050373479844435No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAG510.15741712451385886No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC510.15741712451385886No Hit
CCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAA490.15124390394468795No Hit
GTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGGT480.1481572936601025No Hit
AGCATCCGTGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT480.1481572936601025No Hit
ACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACT470.145070683375517No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATA470.145070683375517No Hit
GCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTGAGGCAATAA460.14198407309093153No Hit
CCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTAC440.1358108525217606No Hit
GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT440.1358108525217606No Hit
ACAGTGTTTAGCTTTGTCACCCAGGACCCATGTACTCTGCGTTGATACCTG430.13272424223717513No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTCTGACTATTTCTGTATGTCTCC430.13272424223717513No Hit
CAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGCGACGTTGCTTTTT420.12963763195258968No Hit
GTTATTGCCTCAAACTTCCGTGGCCTAAACGGCCATAGTCCCTCTAAGAAG410.1265510216680042No Hit
GTATCAACGCAGAGTACATGGGTCCTGGGTGACAAAGCTAAACACTGTCTG410.1265510216680042No Hit
ATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCATG400.12346441138341874No Hit
GATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGA400.12346441138341874No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC390.12037780109883325No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA390.12037780109883325No Hit
ACTAATACTAACATCTCAGACGCTCAGGAAATAGAAACCGTCTGAACTATC390.12037780109883325No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT390.12037780109883325No Hit
CGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAGT370.11420458052966231No Hit
AGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCCTCCACCAGAGT360.11111797024507684No Hit
GAGAACCAAAGTGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAAT360.11111797024507684No Hit
AGGAAGCCAATTGATATCATAGCTCAGACCATACCTATGTATCCAAATGGT360.11111797024507684No Hit
GATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTAGT360.11111797024507684No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAG350.1080313599604914No Hit
ACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCCAAGATTACCCG350.1080313599604914No Hit
CTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCC350.1080313599604914No Hit
GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTG350.1080313599604914No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC350.1080313599604914No Hit
GAGTACATGGGCAGAAATCACATTGCGTTAGCATCCGTGGGGACCATCGCA340.10494474967590592No Hit
CCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATG340.10494474967590592No Hit
GTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCCC340.10494474967590592No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCT330.10185813939132045No Hit
AGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGCATACAGGACTAG330.10185813939132045No Hit
CCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTTCGAGCCT330.10185813939132045No Hit
GATCTTCTGTGAAGGGTTCGAGTTGGAGCACGCCTGTCGGGACCCGAAAGA330.10185813939132045No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)