FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32398
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCAGAACTGGTACGGACAAGGGGAATCCGACTGTTTAATTAAAACAAAG2080.6420149391937774No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC1050.32409407988147415No Hit
GCGCTTACTAGGCATTCCTCGTTGAAGACCAACAATTGCAATGATCTATCC880.2716217050435212No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC710.21914933020556826No Hit
CCTAAGATGAGCTCAACGAGAACAGAAATCTCGTGTGGAACAAAAGGGTAA580.17902339650595717No Hit
ACTCTGGTGGAGGCTCGAAGCGATACTGACGTGCAAATCGTTCGTCTGACT520.16050373479844435No Hit
GGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTT520.16050373479844435No Hit
ACAGATGACCGGCCCAGCAGTCTCTTATACACATCTGACGCTGCCGACGAA500.1543305142292734No Hit
GTCCTGTATGCCCTTTTCCTAACACTCACAACAAAACTAACTAATACTAAC480.1481572936601025No Hit
GCCTGATACTGGCATTTTGTAGATGTGGTCTGACTATTTCTGTATGTCTCC480.1481572936601025No Hit
GTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAACTCCGAAGGT470.145070683375517No Hit
CATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCAT460.14198407309093153No Hit
GAGAACCAAAGTGAACGAAAATCTGTTCGCTTCATTCATTGCCCCCACAAT450.13889746280634607No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC420.12963763195258968No Hit
AGCATCCGTGGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT420.12963763195258968No Hit
GCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCT410.1265510216680042No Hit
GCTTCTTAGAGGGACTATGGCCGTTTAGGCCACGGAAGTTTGAGGCAATAA410.1265510216680042No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT410.1265510216680042No Hit
GTATCAACGCAGAGTACATGGGTCCTGGGTGACAAAGCTAAACACTGTCTG400.12346441138341874No Hit
ACAGTGTTTAGCTTTGTCACCCAGGACCCATGTACTCTGCGTTGATACCTG400.12346441138341874No Hit
GAACATCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCGTGGCCT400.12346441138341874No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC400.12346441138341874No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT390.12037780109883325No Hit
GGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATTGCAATTGTTG390.12037780109883325No Hit
GTTTAGGCCACGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGAT380.11729119081424778No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC380.11729119081424778No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCAG370.11420458052966231No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT370.11420458052966231No Hit
TAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTATAT360.11111797024507684No Hit
ATACAGGACTAGGAAGCAGATAAGGAAAATGATTATGAGGGCGTGATCATG350.1080313599604914No Hit
GATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGA340.10494474967590592No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCT330.10185813939132045No Hit
GACAGGCCCGGGTAATCTTGGGAAATTTCATCGTGATGGGGATAGATCATT330.10185813939132045No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)