FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4923
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCCGGTCATCTGTCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1062.1531586431037986No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT180.3656307129798903RNA PCR Primer, Index 10 (95% over 21bp)
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGC140.28437944342880356TruSeq Adapter, Index 10 (96% over 25bp)
CTGGGCCGGTCATCTCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG120.2437538086532602No Hit
CTGGGCCGGTCATCTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACC80.16250253910217347No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC70.14218972171440178TruSeq Adapter, Index 10 (95% over 22bp)
CTGGGCCGGTCATCTGTCTCTTATACACATCTCTGTCTCTTATACACATCT70.14218972171440178No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCTGCTGTCTCTTATACACATC70.14218972171440178No Hit
CTGGGCCGGTCATCTGTCTCTTATACACATCCTGTCTCTTATACACATCTC60.1218769043266301No Hit
GGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACATGGCTGTCTC50.10156408693885843No Hit
ATGTACTCTGCGTTGATACCCCATGTACTCTGCGTTGATACCTGTCTCTTA50.10156408693885843No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)