FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2592844
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT48800.18821032040492988Clontech Universal Primer Mix Long (96% over 25bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG48090.18547201451379258No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC40580.15650768036950932Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG38020.146634352085972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)