FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences977037
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT140911.442217643753512Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC100581.0294390079393103Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG89700.9180819150144774No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG61620.6306823590099454Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT46740.4783851583921591Clontech SMART CDS Primer II A (96% over 26bp)
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC39650.4058188175063994No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA34630.3544389823517431No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA32740.33509478146682264Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT23860.24420774238846635Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA21530.22036012965732107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCACTTTGGGAGGCCGAGGTGGGTGGATCACAAGGTCAGGAGATCGAGACC20840.21329796108028662No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG19830.2029605838878159No Hit
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC18940.1938514099261338Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA18430.18863154619528227No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG16500.1688779442334323Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCGAGTAGCTGGGATTACGGGCGCCCGCCACCACGCCCGGCTAATTTTTT14790.15137604819469477No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT14590.1493290428100471No Hit
CCTAGTTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT12010.12292267334809225No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC11390.11657695665568449Clontech SMART CDS Primer II A (96% over 26bp)
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG10750.11002653942481196No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC10730.1098218388863472No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG9880.10112206600159461No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)