FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831418
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG40550.4877209778955952No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT32850.395108116494952Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC31530.37923162596912746Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG14780.17776858331188403Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT14220.17103310248274634Clontech SMART CDS Primer II A (96% over 26bp)
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC12820.15419440040990212No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA12630.1519091479857304No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA8440.10151331821057517Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT8410.1011524888804428Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)