FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200529
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG45012.2445631305197753No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC19720.9833989098833585No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA19080.9514833266011399No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA14310.713612494950855No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT10430.5201242713024051No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC8130.40542764388193225No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT7500.37401074158849845No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA7170.3575542689586045No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA6810.3396017533623566No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCT5480.2732771818539962No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC5370.2677916909773649No Hit
GGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA4760.23737215066150036No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG4580.22839589286337636No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACA4240.21144073924469778No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC4230.2109420582559131No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG4080.2034618434241431No Hit
CCTAGTTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT3980.19847503353629647No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT3970.1979763525475118No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG3910.19498426661480384No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG3600.17952515596247923No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA3540.17653307002977126No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTC3520.17553570805220192No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC3500.1745383460746326No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA3450.17204494113070928No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT3420.17054889816435526No Hit
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA3310.16506340728772395No Hit
AGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG3300.1645647262989393No Hit
GCGTAGGGATGGTAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGT3290.16406604531015465No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT3260.16257000234380065No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTTT3140.15658583047838467No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCT3000.14960429663539937No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTAA2990.1491056156466147No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT2980.14860693465783004No Hit
CCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATC2740.1366385909269981No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC2660.1326491430167208No Hit
CCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCC2520.12566760917373546No Hit
GCTAGGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAG2520.12566760917373546No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT2510.1251689281849508No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC2500.12467024719616612No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTC2480.12367288521859682No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGAT2470.12317420422981215No Hit
GGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA2450.1221768422522428No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA2380.11868607533075018No Hit
CCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCA2340.1166913513756115No Hit
CCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA2330.11619267038682683No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC2280.11369926544290351No Hit
CAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTT2190.10921113654384154No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC2100.10472300764477954No Hit
GCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTA2090.1042243266559949No Hit
GCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAGACTGTTCAACCT2090.1042243266559949No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC2060.1027282836896409No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCT2060.1027282836896409No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATA2030.10123224072328692No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC2020.10073355973450225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)