FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684818
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT329681.9567692178027538Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC316821.8804404986176546Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG143220.8500621432107207Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT132840.7884531148171494Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA77760.46153353062467284Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT71600.42497171801345907Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG51760.307214191681238No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA48080.2853720698615518Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC43470.2580100639950428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG38260.2270868426144545Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC23670.14048995203042702No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC22040.13081531655051168Clontech SMART CDS Primer II A (96% over 26bp)
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA21930.13016242703959716No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)