FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230612
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC32331.4019218427488596No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG28671.2432137096074791No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA27831.2067888921651952No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA22320.9678594348949752No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT16630.7211246596014085No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT13220.5732572459368983No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC12470.5407350875062876No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC11150.4834960886684127No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA10480.45444296047040045No Hit
CCTAGTTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT9340.4050092796558722No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA9330.4045756508767974No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG9200.3989384767488249No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCT8470.36728357587636373No Hit
GGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA8260.35817737151579276No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT8220.3564428563994935No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACA7100.30787643314311486No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCT6930.3005047438988431No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG6920.30007111511976825No Hit
GCGTAGGGATGGTAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGT6900.29920385756161866No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTC6850.2970357136662446No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC6660.2887967668638232No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG6420.2783896761660278No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG6270.27188524447990564No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA5810.2519383206424644No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC5790.2510710630843148No Hit
AGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG5710.24760203285171628No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA5700.24716840407264148No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT5430.23546042703762163No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCT5340.23155776802594835No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCACTTTGGTT5090.2207170485490781No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC5050.21898253343277888No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA4890.21204447296758194No Hit
CCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCC4890.21204447296758194No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC4860.21074358663035747No Hit
CAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTT4740.20554004128145978No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGAT4700.20380552616516054No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT4670.2025046398279361No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC4650.20163738226978647No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCC4630.20077012471163688No Hit
CCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATC4590.19903560959533764No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATC4480.19426569302551472No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA4370.1894957764556918No Hit
CCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCA4260.1847258598858689No Hit
GCTAGGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAG4250.1842922311067941No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT4120.17865505697882159No Hit
GATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTT4050.1756196555252979No Hit
GCTCATAAGGGGATGGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGT4050.1756196555252979No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTC3930.17041611017640018No Hit
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTTT3920.16998248139732539No Hit
GGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGG3910.1695488526182506No Hit
GATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCA3810.16521256482750246No Hit
GGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA3720.16130990581582919No Hit
CCAACTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT3670.15914176192045512No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCT3540.1535045877924826No Hit
GCTATCACCAGGCTCGGTAGGTTTGTCGCCTCTACCTATAAATCTTCCCAC3370.14613289854821085No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGG3350.14526564099006123No Hit
GCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAGACTGTTCAACCT3320.14396475465283678No Hit
CCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA3260.14136298197838795No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACGG3150.13659306540856506No Hit
GCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTA3110.1348585502922658No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTAA3080.13355766395504137No Hit
GGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT3080.13355766395504137No Hit
GGCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC3000.1300886337224429No Hit
ACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACG2970.12878774738521848No Hit
CCCAACTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC2950.12792048982706886No Hit
GGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTT2910.1261859747107696No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC2910.1261859747107696No Hit
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA2910.1261859747107696No Hit
AATGTGGGGACAGCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATA2890.12531871715262No Hit
GTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCC2890.12531871715262No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCC2830.12271694447817112No Hit
GCATGAGTAGGTGGCCTGCAGTAATGTTAGCGGTTAGGCGTACGGCCAGGG2780.12054880058279707No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAG2770.12011517180372228No Hit
CCACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCT2690.1166461415711238No Hit
GGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTA2680.11621251279204899No Hit
TGGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAG2670.11577888401297418No Hit
ATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTT2670.11577888401297418No Hit
ACTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT2630.11404436889667494No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTA2610.11317711133852532No Hit
CCTTTAATAGCGGCTGCACCATCGGGATGTCCTGATCCAACATCGAGGTCG2610.11317711133852532No Hit
GTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAA2580.11187622500130089No Hit
CTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCC2580.11187622500130089No Hit
GCTATTCCTAGTTTTATTGCTATAGCCATTATGATTATTAATGATGAGTAT2580.11187622500130089No Hit
GACGAAGAGGGGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGAT2520.10927445232685201No Hit
GGCCCAACCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCACAGC2460.10667267965240317No Hit
GTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAG2440.10580542209425355No Hit
GATTTAGCGGGGTGATGCCTGTTGGGGGCCAGTGCCCTCCTAGTTGGGGGG2380.10320364941980469No Hit
GCATGAACGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAAAACAC2370.10277002064072989No Hit
GCCGGAGGAGGAGACCCCATTCTATACCAACACCTATTCTGATTTTTCGGT2360.10233639186165507No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)