FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183458
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG50382.746132629811728No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG40022.181425721418526No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC37342.035343239324532No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA32571.7753382245527587No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA19761.0770857634990023No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT15650.8530562853623173No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC12890.7026131321610396No Hit
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACAAGCAGTGGT12360.673723686075287No Hit
CCTCCGGCGGGGTCGAAGAAGGTGGTGTTGAGGTTGCGGTCTGTTAGTAGT11380.6203054650110652No Hit
CCTAGTTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT9390.5118337712173904No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC8540.4655016407025041Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT8360.4556901307111164Clontech Universal Primer Mix Long (96% over 25bp)
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGA8250.4496942079386017No Hit
CCTCATACTAGGCCTACTAACCAACACACTAACCATATACCAATGATGGCG7540.4109932518614615No Hit
GGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTTTA7120.38809972854822355No Hit
ACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAA6990.38101363799888804No Hit
GTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTATG6680.36411603745816484No Hit
GCCGTTTACCGGGGCTTCAGTTCAATGCTCTCACATCTCCCTTTAACCTTC6180.3368618430376435No Hit
ATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCC5890.3210544102737411No Hit
ATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC5820.31723882305486817No Hit
CCCTTACCATCAAATCAATTGGCCACCAATGGTACTGAACCTACGAGTACA5800.3161486552780473No Hit
CCTATGATGGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCT5680.3096076486171222No Hit
GCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCT5520.30088630640255537No Hit
GCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAGGTCG5090.277447699200907No Hit
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG4930.26872635698634023No Hit
GATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACATTGGAAC4790.26109518254859426No Hit
CCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCT4730.2578246792181317No Hit
CACAGGTGGACTGGTTGAGTATACCGAGGCGCTTGAGAGAACCATGCTGAA4610.25128367255720657No Hit
GCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCC4410.24038199478899802No Hit
ACAGTGGGGAGGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAA4370.23820165923535633No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT4330.2360213236817146No Hit
GCGTAGGGATGGTAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGT4140.22566472980191649No Hit
TATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCC4130.22511964591350608No Hit
GCTATAGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTA4100.2234843942482748No Hit
AGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG4080.22239422647145393No Hit
CCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATC4080.22239422647145393No Hit
ACAATATGGGAGATTATTCCGAAGCCTGGTAGGATAAGAATATAAACTTCA4000.21803355536417057No Hit
CCACAATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTC3920.21367288425688716No Hit
CCATTCGTATAATAATTACATCACAAGACGTCTTGCACTCATGAGCTGTCC3900.2125827164800663No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG3860.2104023809264246Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGGGGATTATGGACCGTACCCGAAACCGACACAGGTGGACTG3840.20931221314960374No Hit
CCTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCTATCTCT3590.19568511593934307No Hit
GGCACAGACCAGGGGGTGGCGACTGTTTAGTAAAAACACAGGGCTCTGCGA3590.19568511593934307No Hit
GGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA3570.1945949481625222No Hit
GCTAGGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAG3550.19350478038570135No Hit
GCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCT3540.19295969649729094No Hit
GCACAGACCAGGGGGTGGCGACTGTTTAGTAAAAACACAGGGCTCTGCGAA3540.19295969649729094No Hit
GGTAGGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGT3530.19241461260888051No Hit
CATAATCATTTTCCTTATCTGCTTCCTAGTCCTGTATGCCCTTTTCCTAAC3500.19077936094364922No Hit
GGTAAGGGAGGGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGA3500.19077936094364922No Hit
CCAACTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCT3420.1864186898363658No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT3380.18423835428272411Clontech SMART CDS Primer II A (96% over 26bp)
GTTACTACGAGGGCTATGTGGCTGATTGAAGAGTATGCAATGAGCGATTTT3290.17933259928703027No Hit
CAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCTT3230.17606209595656772No Hit
CCCTACGCATCCTTTACATAACAGACGAGGTCAACGATCCCTCCCTTACCA3140.17115634096087387No Hit
GATTGAAGCCCCCATTCGTATAATAATTACATCACAAGACGTCTTGCACTC3080.16788583763041132No Hit
GGTCTGTGCCACCCCACAACGCTTGCGCGCCATGGGGTCTCGCTTATCCCG3070.1673407537420009No Hit
AGGATTGTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCACTTTGGTT3060.16679566985359046No Hit
GACAGTGGGGAGGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACA2990.16298008263471747No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAGACCACATCTACAAAATGC2980.16243499874630704No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGCT2950.16079974708107578No Hit
ACTAATCACCACCCAACAATGACTAATCAAACTAACCTCAAAACAAATGAT2900.15807432763902365No Hit
GCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAGACTGTTCAACCT2900.15807432763902365No Hit
CCCAACTAGGAGGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCC2800.15262348875491938No Hit
GATTATGGACCGTACCCGAAACCGACACAGGTGGACTGGTTGAGTATACCG2630.14335706265194212No Hit
GTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCC2630.14335706265194212No Hit
CCACTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCT2590.14117672709830043No Hit
GCTCATAAGGGGATGGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGT2580.14063164320989No Hit
GCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTA2450.13354555266055446No Hit
AGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAA2450.13354555266055446No Hit
GCTCACGTACCGCTTTAATCGGCGAACAGCCGAACCCTTGGGACCTGCTCC2440.13300046877214405No Hit
CCGAAAGGTATCGTTATCTGAATACATAGGGTAACGAGGCGAACCGGGTGA2440.13300046877214405No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATC2430.13245538488373362No Hit
CCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAATGGC2410.13136521710691276No Hit
GGTAATGATGTCGGGGTTGAGGGATAGGAGGAGAATGGGGGATAGGTGTAT2320.12645946211121892No Hit
CCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAGACTGTTCAACCTGTT2270.12373404266916678No Hit
AGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAG2270.12373404266916678No Hit
GTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTAGAG2240.1220987910039355No Hit
GCTTACTGGTTGTCCTCCGATTCAGGTTAGAATGAGGAGGTCTGCGGCTAG2240.1220987910039355No Hit
CCCCACATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCGA2210.12046353933870423No Hit
GCTATGGCCAGCCTTTCCAAGCTGTTCCGTTATGTCTTGTGCTATCACTAA2200.1199184554502938No Hit
GGCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGC2200.1199184554502938No Hit
GGCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGG2160.11773811989665209No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGG2150.11719303600824166No Hit
GATGTGGGGAGGGGTGTTTAAGGGGTTGGCTAGGGTGTAATTGTCTGGGTC2150.11719303600824166No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA2140.11664795211983124Clontech Universal Primer Mix Long (96% over 25bp)
GATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCA2120.11555778434301038No Hit
CCTAGGAATCACCTCCCATTCCGATAAAATCACCTTCCACCCTTACTACAC2120.11555778434301038No Hit
GACGAAGAGGGGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGAT2120.11555778434301038No Hit
CCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGCTGACGCCATAA2110.11501270045459995No Hit
CCTAGCCTTACTACTAATAATTATTACATTTTGACTACCACAACTCAACGG2090.1139225326777791No Hit
GCAATAATGAAGGGTAAGATGAAGTGAAAGGTAAAGAATCGTGTGAGGGTG2080.11337744878936867No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTA2050.1117421971241374No Hit
ACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACG2050.1117421971241374No Hit
CCGGCGTCAAAGTATTTAGCTGACTCGCCACACTCCACGGAAGCAATATGA2050.1117421971241374No Hit
GGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGGGAATTGCATCT2000.10901677768208529No Hit
GCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACC1980.10792660990526443No Hit
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT1960.10683644212844358No Hit
TCATTATTGCAGCCCTAGCAGCACTCCACCTCCTATTCTTGCACGAAACGG1850.10084051935592889No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT1840.10029543546751846Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)