FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2347289
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG104920.44698373314917766No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT92380.3935604009561669Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC90160.38410268185979657Clontech SMART CDS Primer II A (96% over 26bp)
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC46330.1973766332138906No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG40910.17428616587049997Clontech Universal Primer Mix Long (96% over 25bp)
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA40840.17398794950259638No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT31550.13441037724796565Clontech SMART CDS Primer II A (96% over 26bp)
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA26170.11149031925766278No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG23640.10071192767486237No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)