FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3049138
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC113760.37308905008563076No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA94710.31061237635029965No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG68190.22363697543371272No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT55390.18165789806824093Clontech Universal Primer Mix Long (96% over 25bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG54130.1775255826400773No Hit
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT52490.17214701335262622No Hit
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA52150.1710319441101059No Hit
CCTGATAAAGGGGAATTTCCATGCCGTCTACAGGGATGACCTGAAGAAATT46770.15338761315493102No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC42120.13813740145575568Clontech SMART CDS Primer II A (96% over 26bp)
GTTCTTGGGTGGGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACG38670.12682272825959337No Hit
CCTGATTAGTGGCTGTGGCCGTGGCCACCATGACTGTGGCCGTGGCCGTGG36220.11878767048260853No Hit
GCACTTTGGGAGGCCGAGGTGGGTGGATCACAAGGTCAGGAGATCGAGACC33670.11042465116370595No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATC32400.10625953958135054No Hit
CCTAGTTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAAT32360.10612835496458344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)