FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1948555
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG143280.7353141173844208No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT34910.17915840199532473Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC33440.1716143501209871Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG32040.16442953881209407No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC28370.1455950691666389No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA25230.12948056380240744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)