FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216596

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216596
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1322916
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT23542217.795687708063095Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG1128128.527525557178233Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC808656.112633001641828Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA461123.4856332526025837Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA406573.0732865881129263Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC323452.4449776100674567Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG318132.40476341657369Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT288892.183736533536521Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT165411.2503439371811966Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT85150.6436538676680907Clontech Universal Primer Mix Long (96% over 25bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG83990.634885359312307No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA78070.5901357304621004Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGAGCGTGCCAGTCCTGTCTCTTATACACATCTCCGAGCCCAC63970.4835529995857636No Hit
GACTGGCACGCTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC62890.47538921594417183Clontech SMART CDS Primer II A (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG59180.4473451073235187Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG47020.3554269507663374Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAGCGTGCCAGTC45990.34764112007111564Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA25770.1947969485590922Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC22660.1712882752948789Clontech SMART CDS Primer II A (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC21250.16063000220724521Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG18480.13969140897834784No Hit
GAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT16970.12827722999797417Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA16790.12691659939104222Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC16480.12457329112354827Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)