FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070224
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG58290.5446523344645607No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT16190.15127674206521252Clontech Universal Primer Mix Long (96% over 25bp)
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC15270.14268041082988236No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC14950.13969038257411534Clontech SMART CDS Primer II A (96% over 26bp)
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA14190.13258906546666865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)