FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1097142
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG92050.8389980513005608No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT64280.5858858743900061Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC55950.5099613359072936Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT30000.2734377136232138Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG27940.2546616572877531No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG27710.25256530148330847Clontech Universal Primer Mix Long (96% over 25bp)
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC23460.21382829205335319No Hit
CCTGATAAAGGGGAATTTCCATGCCGTCTACAGGGATGACCTGAAGAAATT21780.1985157800904532No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA21040.19177098315441393No Hit
GCACTTTGGGAGGCCGAGGTGGGTGGATCACAAGGTCAGGAGATCGAGACC20800.18958348144542822No Hit
CCTGATTAGTGGCTGTGGCCGTGGCCACCATGACTGTGGCCGTGGCCGTGG17720.16151054284677827No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA16070.14647146859750151Clontech Universal Primer Mix Long (96% over 25bp)
CCCGAGTAGCTGGGATTACGGGCGCCCGCCACCACGCCCGGCTAATTTTTT14610.1331641665345051No Hit
GCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGA13390.12204436618049441No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT13300.12122405303962476Clontech SMART CDS Primer II A (96% over 26bp)
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGG12730.1160287364807837No Hit
GAACCAGGGGGAATTCAAAGAGCTGGTGCGAAAAGATCTGCAAAATTTTCT11390.10381518527228016No Hit
CCCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCGTGCA11000.10026049499517838No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)