FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327718
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT1212129.12934825015553Clontech Universal Primer Mix Long (96% over 25bp)
GGTTTCCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCA684595.156140083963613No Hit
GGTTTCCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAG649174.889366567298176No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG547254.121733681399213Clontech Universal Primer Mix Long (96% over 25bp)
ACGAGACAAGCAGTGGTATCAACGCAGAGTACATGGAGCGTGCCAGTCCTG326212.4569223283859976Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA288142.1701897541496007Clontech Universal Primer Mix Long (96% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC277202.0877927391208075Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAGCGTGCCAGTCCTGTCTCTTATACACATCTCCGAGCCCAC267562.015186959881541No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA203841.535265771797927Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG177681.3382359808332793Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC169181.2742163622094451Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTGGCACGCTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTC140741.0600142500139336Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAA128850.9704621011389467Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT121240.9131457131710198Clontech SMART CDS Primer II A (96% over 26bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCA104500.7870647230812566Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGAGACAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAA89970.6776288338336905Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG60470.45544309860979515No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC54380.4095749247957774Clontech Universal Primer Mix Long (96% over 25bp)
GACTGGCACGCTCCATGTACTCTGCGTTGATACCACTGCTTGTCTCGTCTG52170.39292982395358045Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT51410.3872057168766259Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG47490.3576813751112812No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAGCGTGCCAGTC47170.35527122476308975Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT46600.3509781444553738Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAG46020.3466097469492769Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC40470.30480870184783215Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCA37840.285000278673634Clontech Universal Primer Mix Long (96% over 25bp)
GGTTTCCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGCG34710.2614259955803868No Hit
GAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCA33790.2544968133293365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGGA33020.24869738905400093Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTACATGGAGCGTGCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACG32740.24658850749933345No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG31960.2407137660256169Clontech Universal Primer Mix Long (96% over 25bp)
GACTGGCACGCTCCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCT30590.2303953098474224Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGAGCGTGCCAGTCCTGTCTCTTATACACAT28410.21397616060036842No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGC24910.18761514116702493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG24240.18256888887549916Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGATACCA20540.154701525474536Clontech SMART CDS Primer II A (96% over 26bp)
CGAGACAAGCAGTGGTATCAACGCAGAGTACATGGAGCGTGCCAGTCCTGT20370.15342113310205932Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGAGACAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAAC20220.1522913751263446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACAAGCAGTGGT19310.14543751007367528No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGA18030.13579690868090966Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCATGTACTCTGCGTTGATACCACTGCTTCATGTACTCTGCGTTGA17560.13225700035700352Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACACATCTCCGAGCCCACGAGACAAGAGGCAAT16780.12638225888328697No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC16250.12239044736909493No Hit
ACGAGACAAGCAGTGGTATCAACGCAGAGTACATGGGGCGTGCCAGTCCTG15650.11787141546623606Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCACGAGACAAGCAGTGGTATCAACGCAGAGTACATGGAGCGTGCCAGT15580.11734419507756919Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTAC14550.10958652364432808Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA13470.10145226621918207No Hit
TATAAGAGACAGGGTTTCCCAGTCCTGTCTCTTATACACATCTCCGAGCCC13380.10077441143375326No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)