FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173076
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT368511.6957989504278725Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGTGGAAGCAGTGGTATCAACGCAGAGTACATGGTGGAAGCAG200530.9227933123369823Clontech Universal Primer Mix Long (96% over 25bp)
GTTTCAAGGGCGCACTTTGCGCGATCATCGAAAGGGAATCGGGTTAATATT183320.8435968185190025No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG169440.7797242250156Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGTGGAAGCAGTGGTATCAACGCAGAGTACA162690.7486622649184842Clontech Universal Primer Mix Long (96% over 25bp)
CCGAAGGGGGCGTCCTGCGTCCGTCGCCCGATCGGCACGCGACGGCCCGCC127080.5847931687617001No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC122370.5631188232717126Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG115780.532793146673195No Hit
CCTTGAAACAGGGTTTCCCCATCTCTTAGGATCGACTAACCCATGTCCAAG114340.5261665951858103No Hit
TCCGAAGGGGGCGTCCTGCGTCCGTCGCCCGATCGGCACGCGACGGCCCGC108100.4974515387404766No Hit
ACGAGTTCGGGGCGCAGACCCCTAGGCCCAGCCCTCAGAGCCAATCCTTTT107880.496439148929904No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCACCATGTACTCTGCGTTGAT90800.41784088545453546Clontech SMART CDS Primer II A (96% over 26bp)
CCGAACTCGTGGGCTGTTGGCGGCCTGCCCGAGCTGCTCCCGCGGCGAGGG80750.3715930781988297No Hit
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA78530.36137714465577825Clontech Universal Primer Mix Long (96% over 25bp)
CCCTGAAATCGGTTCAACCGGAGATAGGGTCCAGCGGCTGGAAGAGCACCG78480.3611470560624663No Hit
CCTTTCACCCTCTCTATCATCCATATCTAGATTCCAATGTTGTAGATCAGG67530.3107576541271451No Hit
GTATCAACGCAGAGTACATGGTGGAAGCAGTGGTATCAACGCAGAGTACAT65020.29920720674288426Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCACCATGTACTCTGCGTTGATACCACTGCTTCCACCATGTACTC63200.29083198194632864Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA59760.27500188672646514Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCGTGAGCCTGGGTGGACCGGCCCCCGGTGCAGATCTTGGTGGTAGTAG56920.2619328546263453No Hit
ATTTCAGGGTGGGCAGGCTGTTAAAAAGAAAAGATAACTCTTCCCGGGGCC56590.26041426991048633No Hit
CCCTTGAAACAGGGTTTCCCCATCTCTTAGGATCGACTAACCCATGTCCAA54620.25134877933399474No Hit
GATCCCACAAAGGGTGTTGGTTGATTAAGACAGCAGGACGGTGGTCATGGA53770.24743727324769127No Hit
AGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC53410.24578063537584513Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCAGAGTACATGAA51160.23542664867680654Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTTCACCCTCTCTTTCTTCCATGTCTAGGTCCAATGTTGTAGATCAGGG50490.23234346152642615No Hit
GCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAA50020.23018062874929363No Hit
AGCATCCGCAGGGACCATCGCAATGCTTTGTTTTAATTAAACAGTCGGATT48820.22465850250980635No Hit
GCTGAGTTTAAGCATATCACTAAGCGGAGGAAAAGAAACTTACCAGGATTC47560.21886026995834476No Hit
CCCTTCGGAGGGCTTTCCCTAGGCGGCGAACAGTCGACTCAGAACTGGTAC47370.2179859333037593No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG47210.21724964980516098Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCGATTAGGGCTGCATTCCCAAACAACCCGACTCGCCGACAGCGCCTCGT44830.20629743276351126No Hit
CCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCCTAGGGGTCTGC43380.19962486355746414No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGTGGAAGCAGTGGTATCAACG42650.19626557009510942Clontech Universal Primer Mix Long (96% over 25bp)
GCAATTTGGCAAGATTTCCCAAACCTTTCGGGCCAGGGAGAGAAACTCGTT42090.19368857785001536No Hit
CCTTGTACGGCGGTTTTAGTTACAGTTCCCACTCCCAGTGAAAGTGAAAGC41140.18931689457708795No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT40690.18724609723728022Clontech SMART CDS Primer II A (96% over 26bp)
GGTGGAAGCAGTGGTATCAACGCAGAGTACATGGTGGAAGCAGTGGTATCA40450.1861416719893828Clontech Universal Primer Mix Long (96% over 25bp)
TCTAGTAGCTGGTTTCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCACG39290.1808036166245451No Hit
CATGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATCAACGCA37310.17169210832939114Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGAAAATGGATGGCGCTGAAGCGCGCAACCTATACTCGGCCGTCGGGGCAA36870.16966732870824583No Hit
CCAAGGTGAACAGCCTCTGGTCAATAGAACAATGTAGGTAAGGGAAGTCGG33640.15480360558029263No Hit
GGTAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCT32940.15158236527392507No Hit
ACTTGGAGCTCGCGATTCCGTGGCGCGGCTCAACAGAGCAGCCGCGCCGAC32220.1482690895302327No Hit
GGTTTCCCAGTCCATGTACTCTGCGTTGATACCACTGCTTCCACCATGTAC31730.14601422131577543Clontech SMART CDS Primer II A (96% over 26bp)
GCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGGGATGAACC31350.14426554800660446No Hit
GGAGGACGCGGGGGTTGTTGCGAAACCTTGGGCGTGAGCCTGGGTGGACCG29620.13630448267801035No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC29330.13496996883680093No Hit
GCTAGTTAGCAGGCTGAGGTCTCGTTCGTTAACGGAATTAACCAGACAAAT29140.13409563218221543No Hit
GGGTGGACCGGCCCCCGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAA28510.13119651590648462No Hit
CCGATGAGTAGGAGGACGCGGGGGTTGTTGCGAAACCTTGGGCGTGAGCCT28390.1306443032825359No Hit
CCAGGAGTGGAGCCTGCGGCTTAATTTGACCCAACACGGGGAAACTTACCA28380.1305982855638735No Hit
AGTACATGGTGGAAGCAGTGGTATCAACGCAGAGTACATGGTGGAAGCAGT28340.13041421468922393Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTTACGGTGCCAAATTGCGCGCTAACCCAGATCCCACAAAGGGTGTTGGT28230.12990801978393762No Hit
GCGCCTTCGGCGGCCCTTGAAAATCTAGAGGACCGAGTACCGTTCACGCCC28130.12944784259731368No Hit
GCCCTCGCCGCGGGAGCAGCTCGGGCAGGCCGCCAACAGCCCACGAGTTCG27240.12535226563636062No Hit
GCAAGTGCCAGGCTCCGATGAGTAGGAGGACGCGGGGGTTGTTGCGAAACC26980.1241558049511384No Hit
CCATGTGAACAGCACTTGGACATGGGTTAGTCGATCCTAAGAGATGGGGAA26860.12360359232718966No Hit
GCAAAATGGATCCGTAACTTCGGGAAAAGGATTGGCTCTGAGGGCTGGGCC26440.12167084814336912No Hit
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG26010.1196920862408862No Hit
TTGTGGGATCTGGGTTAGCGCGCAATTTGGCACCGTAACTCGGCTTTCGGT25690.11821951924368959No Hit
GCGTTGATACCACTGCTTCCACCATGTACTCTGCGTTGATACCACTGCTTC25590.11775934205706565Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGTGGAAGCAGTGG25490.11729916487044172Clontech Universal Primer Mix Long (96% over 25bp)
CTTGTACGGCGGTTTTAGTTACAGTTCCCACTCCCAGTGAAAGTGAAAGCT25100.11550447384260835No Hit
GGTTTCCCCATCTCTTAGGATCGACTAACCCATGTCCAAGTGCTGTTCACA24920.11467615490668528No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA24520.11283544616018952No Hit
CCTAAGAGATGGGGAAACCCTGTTTCAAGGGCGCACTTTGCGCGATCATCG24430.11242128669222796No Hit
GCTCACGCCCAAGGTTTCGCAACAACCCCCGCGTCCTCCTACTCATCGGAG24160.11117880828834334No Hit
TCTCTTAGGATCGACTAACCCATGTCCAAGTGCTGTTCACATGGAACCTTT23640.10878588691789887No Hit
GCTAACTAGCTACGCGGAGGTTCCCCTTCGCGGCCAGCTTCTTAGAGGGAC23370.10754340851401424No Hit
GTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCGGGCGTGAACGGT23160.10657703642210396No Hit
GAGTACATGGTGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG23080.10620889467280482Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT22430.10321774295974921Clontech SMART CDS Primer II A (96% over 26bp)
CATGGTGGAAGCAGTGGTATCAACGCAGAGTACATGGTGGAAGCAGTGGTA22280.10252747717981331Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTTTCCCAGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGA21890.10073278615197996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)