FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216617

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216617
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537378
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC97740.6357577641933214Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT96810.6297085036991553Clontech Universal Primer Mix Long (96% over 25bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG94500.614682921181388No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG60920.3962590852737583Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC51830.3371324423791676Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG50040.3254892420731922Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG43090.2802824028963599Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT42670.2775504788022204Clontech SMART CDS Primer II A (96% over 26bp)
GGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGG39640.25784159783735683No Hit
TCATAATCGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCC36110.23488042628423197No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATA31260.20333320757809728No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTG27340.17783524936612857Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA26380.17159085143666686Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG25960.16885892734252733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACCA21930.14264546520114116No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCAT20150.1310673107069309Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG19740.12840043242455662Clontech SMART CDS Primer II A (96% over 26bp)
CCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCT18320.1191639271538945No Hit
CCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG15810.10283742840082269Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA15700.10212192447140521Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)