FastQCFastQC Report
Thu 7 Jul 2022
EGAF00006216625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28809469
Sequences flagged as poor quality0
Sequence length90
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG26678489.260316460535945No Hit
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCACTTGGCGAGG17012365.905127928598754No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG6138742.1308063678646767No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACATTGCCAAG3379071.172902562001403No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAAG3344101.1607641918009666No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG3164671.098482585708192No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAGG2305000.8000841667716958No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCCAAG2031040.7049904321388222No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1865210.6474294961840498No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCACATTGCCAAG1798130.6241454849445507No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAAG1653300.5738738190558111No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCGAGG1551200.5384340822109565No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG1504440.5222033075305901No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAAG1428250.4957571415148262No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCCAGG1413370.4905921730108944No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAAG1290070.4477937444803304No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAAG1189690.4129510335646936No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAAG1166300.4048321751435266No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCGAAG1104550.38339825006840633No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCCCATTGCCAAG1078660.37441162140128303No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCCAAG1024390.35557406490206395No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAGG1017370.3531373660514187No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCGAGG972690.337628576215688No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAAG970860.336993368395648No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAGG811630.2817233458902002No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCACATTGCCAAG786740.2730838253214594No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCACATTGCCAAG773420.26846034545100433No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCCCATTGCCAAG769700.26716910332502136No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCACATTGCCAAG762030.2645067842104275No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCACATTGCCAAG731010.2537394909986019No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCACATTGCCAAG699990.2429721977867763No Hit
GTTTACCACTACACAAAAAAGACGTCAACAAAAATAACCCCATTGCCAAG672620.23347184913404687No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCCCATTGCCAGG669450.2323715164621743No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCGAGG668350.23198969755395352No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCGAAG668260.23195845782509908No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCACATTGCCAAG644880.22384307048491592No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCACATTGCCAGG634560.22026091490960834No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACAATGCCAAG616600.21402685346265843No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAACAATAACCACATTGCCAGG615660.21370057185017885No Hit
GTTTACCACTACACAAAAAATACCTAAACAAAAATAACCCCATTGCCAAG615300.21357561293476116No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAGG591170.20519989452079107No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCACATTGCCAAG582110.20205509514944547No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCCCATTGCCAAG560510.19455756022438317No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCGAGG526130.18262398380199232No Hit
GTTTACCACTACACAAAAAATACCTCAACAAAAATAACCCCATTGCCAAG509680.17691405558359996No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCGAGG508810.17661207153800718No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAGCCCCATTGCGAGG503950.17492512617986816No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATAACCCCATTGCCAAG501450.17405735593391186No Hit
GTTTACCACTACACAAAAAAGACGTCAACAAAAATAACCCCATTGCCAGG499480.17337355298009832No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAGG494580.171672723298024No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATATCCCCATTGCCAAG484810.16828147717682684No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCCCATTGCCAGG484540.16818775799026356No Hit
GTTTACCACTACACAAAAAAGAAGTCACCAAAAATAACCCCATTGCCAAG475440.16502907429498267No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAGCCCCATTGCGAGG461950.16034658604780255No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCACATTGCCAAG443740.15402574757625695No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCCCATTGCCAAG436790.15161334629249848No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAGG429820.14919400284677237No Hit
GTTTACCACTACACAAAAAAGACGTCAACAAAAATAACCACATTGCCAAG427580.14841648070639551No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCGAAG422200.1465490391370976No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAACCACATTGCGAGG418620.1453063921448882No Hit
GTTTACCACTACAAAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG417640.14496622620847333No Hit
GTTTACCACTCCACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAAG411900.1429738257237577No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCACATTGCCAGG411510.14283845356538852No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCCCATTGCGAGG407480.141439607928907No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAGG407160.14132853333742457No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCCCATTGCCAAG388550.13486885162652598No Hit
GTTTACCACTACACAAAAAATACCTAAACAAAAATAACCACATTGCCAAG387420.13447661947535375No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCGAGG376380.13064454606921078No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCCAGG369240.12816619424675962No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAACCACATTGCCAGG364800.12662503428994126No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAGCCCCATTGCCAGG362580.12585445431153208No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCACATTGCCAAG361520.12548651972724661No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCCCATTGCCAGG349900.12145312362404181No Hit
GTTTACCACTACACAAAAAAGACCTCACCAACAATAACCCCATTGCCAAG346100.12013411285018824No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAGG341370.11849229154483895No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCACATTGCGAGG329540.11438600274097381No Hit
GTTTACCACTACACAAAAAATACCTCAACAAAAATAACCACATTGCCAAG328020.11385839843143239No Hit
GTTTACCACTACACAAAAAAGACCTAAACAAAAATAACCCCATTGCCAAG325510.11298715710449227No Hit
GTTTACCACTACACAAAAAAGACGTCAACAAAAATAACCACATTGCCAGG318430.11052963176794407No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAGCCACATTGCGAGG316530.1098701263810173No Hit
GTTTACCACTCCACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAGG313230.108724669656355No Hit
GTTTACCACTACACAAAAAAGACGTAAACAAAAATAACCCCATTGCCAAG306210.10628797080570974No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCACATTGCCAGG303510.10535077894007695No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATATCCCCATTGCCAAG300620.10434763653575149No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACAATGCCAAG300580.10433375221181619No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAGCCCCATTGCGAGG298640.10366036250095412No Hit
GTTTACCACTACACAAAAAATACGTCAACAAAAATAACCCCATTGCCAAG297120.1031327581914127No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAACAATAGCCCCATTGCGAGG293720.10195259065691215No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCACATTGCGAGG293480.10186928471330034No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATATCCACATTGCCAAG290890.10097027473848963No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)