FastQCFastQC Report
Thu 7 Jul 2022
EGAF00006216629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13475971
Sequences flagged as poor quality0
Sequence length90
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7112165.277660511439213No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5939704.4076230202632525No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5124523.8027092815797836No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1473431.0933757574871599No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1369601.0163275061960286No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1168450.8670618243390401No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1133190.8408967338976909No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1122430.8329121515622139No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1080590.8018642960867162No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1006230.7466845988315054No Hit
ACGTTGTGGCCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920700.6832160740031276No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT908070.6738438365591615No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG896180.6650207246661484No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT877610.6512406415834525No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG776970.5765595666538612No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA638480.4737914618545854No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT516590.38334157887398246No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT480230.35636022072175727No Hit
TTCTTTGCCTTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469400.3483236940774064No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA467260.34673568234897506No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT454450.33722987382504754No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA445930.330907509373536No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA438530.3254162538640073No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA435880.3234497907423517No Hit
GTCTCTTGGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA428040.3176320281484726No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA419880.3115768058568841No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA403500.2994218375803866No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA385270.2858940554265069No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA366340.27184683018388806No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA355980.26415907247054776No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC353550.2623558628910674No Hit
TTCTTTGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA349990.25971412375405084No Hit
TTCTTTGCCTTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA341420.2533546562247722No Hit
AACCCACCGTTGTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA329560.24455380617841935No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC328580.24382658585418446No Hit
TTCTTTGCCTTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA325810.24177107534588788No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA319520.2371035081627884No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA313280.23247304405745606No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC312660.23201296589314419No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA304480.22594290237044884No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA283740.21055254571266144No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA280360.20804437765560643No Hit
ACGTTGTGGCCTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA272580.20227113875504776No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA263850.1957929413769145No Hit
AACCCACCGTTGTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA255180.18935926769210173No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA249280.18498110451558555No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA243770.18089234534565266No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT243260.18051389395242837No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA238940.1773081880333521No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG237090.1759353741559699No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA232210.1723141137659023No Hit
AACCCACCGTTGTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA226450.16803983920713395No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT223950.1661846853187796No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT223650.1659620668521771No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG223610.16593238438996344No Hit
GAGTCACCAATCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA217980.1617545778333895No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT213130.15815557928998214No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG210950.15653788509933717No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA206910.15353995641575663No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA196050.14548116792474547No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA195270.1449023599115789No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG188460.13984892071970176No Hit
CCTATTCCGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181060.13435766521017298No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180480.1339272695080748No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT179040.1328587008683827No Hit
ACGTTGTGGCCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA177800.13193854453975895No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT177160.13146362514434023No Hit
ACGTTGTGGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170080.1262098293325208No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG169420.12572006870599528No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG166020.12319705941783342No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA164040.1217277775382568No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT162880.1208669861340604No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG162320.12045143166306903No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG160060.11877437254799673No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG158480.11760191529055679No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC157640.11697858358406975No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG157060.11654818788197155No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT156710.11628846633760194No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA155040.11504922354018125No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152990.11352799735173072No Hit
ACGTTGTGGCCTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152530.11318664903627353No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC146820.10894947755527226No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG145690.10811094799773613No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA143320.10635226211157622No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA142450.10570666855842892No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA141820.10523916977856362No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA141730.1051723842385829No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC140940.10458615560986291No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140670.10438579898992065No Hit
ACGTTGTGGCCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT135570.10060128505767785No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGA134990.10017088935557965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)