FastQCFastQC Report
Thu 7 Jul 2022
EGAF00006216631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13534605
Sequences flagged as poor quality0
Sequence length90
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7160795.290726992032646No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5978684.41732876578223No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5165303.816365531169916No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1478921.0926953538725364No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1370501.0125895805603489No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1182210.8734721109334185No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1143820.8451077811284482No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1130750.8354510530599157No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1095180.8091702713156388No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1001130.7396817269510266No Hit
ACGTTGTGGCCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA920460.6800789531722574No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT916390.6770718465740228No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG900290.6651764126104899No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT878790.6492912057647785No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG775840.573226924612872No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA638150.47149510458561594No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT518270.3829221466012492No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT484160.3577200812288205No Hit
TTCTTTGCCTTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA476310.35192013361306074No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469330.3467629827394298No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT456320.3371505854806993No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA449050.33177916902635873No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440700.32560979799558243No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440090.32515910142926224No Hit
GTCTCTTGGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA429090.3170317862988983No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA424710.31379563718335335No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA403980.2984793423967674No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA384220.28387972903531355No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC370670.2738683544883652No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA366730.27095729797803486No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA359050.2652829543233807No Hit
TTCTTTGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA351210.25949039517592126No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC344060.2542076403411847No Hit
TTCTTTGCCTTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA341770.2525156811004089No Hit
AACCCACCGTTGTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330320.24405588489653007No Hit
TTCTTTGCCTTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA326640.24133692856200825No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC326420.241174382259401No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA324240.23956369616992887No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA314290.23221217021109963No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA306220.2262496762927326No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA283980.2098177227927967No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA281470.20796321724941363No Hit
ACGTTGTGGCCTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA274520.20282823178068365No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA268090.1980774466635709No Hit
AACCCACCGTTGTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA256860.1897801967622993No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA247240.18267249025738097No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT245100.18109135804111018No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA242240.17897825610721554No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA241860.17869749431180296No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA236590.17480377151752857No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT230920.17061450999124098No Hit
AACCCACCGTTGTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA227040.16774778429071258No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT223960.16547213605421066No Hit
GAGTCACCAATCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA223680.1652652589418014No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT217520.16071396246879757No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG212720.15716749768463875No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA212350.1568941243575265No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAA204620.15118283836137072No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG200620.14822745104123836No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA195420.14438544752506632No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG193400.14289297692839945No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG189060.13968638168605585No Hit
CCTATTCCGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA188160.13902141953902608No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182370.1347434963931345No Hit
ACGTTGTGGCCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA179550.13265994833244116No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT178250.13169944745339818No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT176640.13050990405704488No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG175240.12947551849499855No Hit
ACGTTGTGGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA169820.1254709686762192No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA167590.12382334024524543No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG166140.12275201234169746No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG165070.12196144623356205No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT162240.1198705097045684No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC158970.11745448057036019No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG158890.11739537282395755No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT157610.11644964888151518No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG157580.11642748347661422No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG157340.11625016023740625No Hit
GTCTCTTGGCTTAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA156920.11593984456879237No Hit
ACGTTGTGGCCTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155740.11506800530935331No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATG149100.11016206235793362No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148320.10958576183050779No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC147530.10900207283478167No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA146270.10807112582893996No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA144680.10689635936918734No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143590.1060910163244513No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTA141990.10490886139639835No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC141090.10424389924936857No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA139780.10327600990202522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)