FastQCFastQC Report
Thu 7 Jul 2022
EGAF00006216639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27467510
Sequences flagged as poor quality0
Sequence length90
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG27379079.96780195947867No Hit
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCACTTGGCGAGG19660257.1576382424180425No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG5179161.8855586108824571No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACATTGCCAAG3227421.1749954764738413No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAAG2822621.0276213606548246No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG2711690.9872354647363376No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAGG1939770.7062052585035921No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCCAAG1936370.7049674324319897No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCACATTGCCAAG1754890.6388966455277526No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1600870.5828231244841633No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAAG1411940.5140400422171504No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCCAGG1351510.4920395041268757No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCGAGG1321930.48127041730393466No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG1250990.455443540386442No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAAG1216770.44298518504225537No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAAG1093660.3981649592554986No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAAG1010110.36774720387832754No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAAG986450.3591333906859413No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCGAAG944490.34385716069640093No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCGAGG942020.34295791646203094No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAAG931110.3389859510381538No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCCCATTGCCAAG920660.3351814562004346No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCCAAG858280.3124709884514468No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAGG854970.3112659283640927No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAACAATAACCCCATTGCCAGG854200.31098559716552393No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCACATTGCCAAG760270.276788831605049No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCACATTGCCAAG732730.26676244042506947No Hit
GTTTACCACTACAAAAAAAATAACTAAACAAAAATAACCACATTGCCAAG723370.26335477806324636No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCACATTGCCAAG698400.25426403776680156No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAGG697490.25393273725940213No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCGAAG652950.2377172157214105No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCCCATTGCCAAG651950.23735314922976275No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCACATTGCCAAG650800.23693447276436777No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCACATTGCCAAG625760.22781824781350768No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCACATTGCCAGG608680.22159999213616377No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACAATGCCAAG602460.21933549855811466No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCCCATTGCCAGG566190.20613080690605007No Hit
GTTTACCACTACACAAAAAAGACGTCAACAAAAATAACCCCATTGCCAAG561100.20427770846356297No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCACATTGCCAAG556530.20261392459673266No Hit
GTTTACCACTACACAAAAAATACCTAAACAAAAATAACCCCATTGCCAAG526030.1915098966014757No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAGG493820.17978331490550106No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAGG470280.1712131896921126No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCCCATTGCCAAG469840.17105300043578758No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCGAGG450670.16407384579089987No Hit
GTTTACCACTACACAAAAAATACCTCAACAAAAATAACCCCATTGCCAAG440660.1604295402095057No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATAACCCCATTGCCAAG429340.15630830752405295No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCGAGG429220.15626461954505522No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAGCCCCATTGCGAGG428760.15609714895889726No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCACATTGCCAAG426310.15520518605436023No Hit
GTTTACCACTACACAAAAAAGACGTCAACAAAAATAACCCCATTGCCAGG421270.15337029093645546No Hit
GTTTACCACTACACAAAAAAGAAGTCACCAAAAATAACCCCATTGCCAAG415310.15120045464623477No Hit
GTTTACCACTACACAAAAAAGACGTCAACAAAAATAACCACATTGCCAAG409570.14911071298417658No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATATCCCCATTGCCAAG407540.14837165800613158No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCCCATTGCCAGG405270.14754522707009116No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCACATTGCCAGG400060.1456484406486063No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAACCACATTGCGAGG395860.14411936138368567No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAGCCCCATTGCGAGG393840.14338394707055718No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCCCATTGCCAAG371170.13513055970490226No Hit
GTTTACCACTACACAAAAAATACCTAAACAAAAATAACCACATTGCCAAG370920.13503954308199034No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAGG364400.13266582955644687No Hit
GTTTACCACTCCACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAAG358020.1303430853397341No Hit
GTTTACCACTACAAAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG352920.1284863462323305No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCGAAG351700.12804218511252022No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAGG349870.12737594343280478No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCACATTGCCAAG346450.12613083603136943No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAACCACATTGCCAGG344930.1255774549640648No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCCCATTGCCAAG329260.11987253303994429No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCGAGG327310.11916260338123114No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCACATTGCGAGG319330.11625735277788195No Hit
GTTTACCACTACACAAAAAATACCTCAACAAAAATAACCACATTGCCAAG314440.11447706763372437No Hit
GTTTACCACTACACAAAAAAGACGTCAACAAAAATAACCACATTGCCAGG311120.11326836688145377No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCCAGG310680.11310817762512873No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAGCCACATTGCGAGG307160.1118266635745286No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAAAAATAGCCCCATTGCCAGG306830.11170652163228482No Hit
GTTTACCACTACACAAAAAAGACCTCACCAACAATAACCCCATTGCCAAG295700.10765446158024516No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCCCATTGCCAGG295340.10752339764325197No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAGG291000.10594334906950066No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCACATTGCCAGG289920.10555015725852107No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACAATGCCAAG286430.10427956520267034No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATATCCACATTGCCAAG280270.10203691561412011No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCACAATGCCAAG278520.1013997992537365No Hit
GTTTACCACTCCACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAGG275030.1001292071978858No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)