FastQCFastQC Report
Thu 7 Jul 2022
EGAF00006216641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27293241
Sequences flagged as poor quality0
Sequence length90
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGG26954089.87573443549632No Hit
GTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCACTTGGCGAGG19421957.11602920298106No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAAG2832111.0376598367339371No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAAG2322060.8507820672524747No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAGG2220120.8134321607316624No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAAG2151150.7881621680620488No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCCCATTGCCAAG2006620.7352076655168949No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCCCATTGCCAAG1896650.6949156386374195No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAAG1830670.6707411552918907No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCACATTGCCAAG1828570.6699717340274832No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAAG1565050.5734203570766843No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCACATTGCCAAG1553840.5693131130890612No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1482020.5429989058463228No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAAG1405390.5149223575170131No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAAG1268750.4648586805795618No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCCAAG1254010.4594580760855773No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACATTGCCAAG1180900.43267122435184596No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCACATTGCCAAG1147800.4205436796604698No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAGG1083960.39715327322248023No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCACATTGCCAAG1069070.3916977100667524No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAGG997030.36530289678679057No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAGG841850.3084463292578555No Hit
GTTTACCAATACACAAAAAAGACCTCACCAAAAATAACCCCATTGCCAAG814600.29846217237447176No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCCCATTGCCAAG791420.28996922717972556No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCACATTGCCAAG776400.28446603318382013No Hit
GTTTACCAATACACAAAAAAGAACTCACCAAAAATAACCCCATTGCCAAG748200.2741338047760616No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAGG737130.27007785553939895No Hit
GTTTACCACTACACAAAAAAGACCTCACCAAAAATAACCACATTGCCAGG710040.26015232122854154No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCCCATTGCCAAG695750.2549165927197873No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCCCATTGCCAAG583740.2138771280406017No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCACATTGCCAGG573080.20997139914603766No Hit
GTTTACCAATACACAAAAAAGACCTCACCAAAAATAACCACATTGCCAAG540770.19813330340651006No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCACATTGCCAAG522260.19135140454737493No Hit
GTTTACCAATACACAAAAAAGAACTCAACAAAAATAACCCCATTGCCAAG521020.19089707961029617No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCCCATTGCCAGG512080.18762154337038975No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCACATTGCCAAG491900.18022777141051147No Hit
GTTTACCACTACACAAAAAATACCTCAACAAAAATAACCCCATTGCCAAG478770.17541705655257284No Hit
GTTTACCAATACACAAAAAAGAACTCACCAAAAATAACCACATTGCCAAG473850.17361441244738945No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCCCATTGCCAAG472210.17301353107899498No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCCCATTGCCAGG459490.16835303656315495No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCCCATTGCCAAG456310.16718791293419494No Hit
GTTTACCAGTACACAAAAAAGACGTCACCAACAATAACCCCATTGCGAGG431530.15810874201418584No Hit
GTTTACCACTACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAGG395880.14504690007317195No Hit
GTTTACCACTACACAAAAAATAACTCACCAAAAATAACCCCATTGCCAGG393070.144017341143179No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATATCCACATTGCCAAG392480.1438011704069883No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCGAGG387710.1420534849635483No Hit
GTTTACCACTACACAAAAAAGACGTCACCAAAAATAACCACATTGCGAGG379380.13900144728139835No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATATCCCCATTGCCAGG367620.13469268820071606No Hit
GTTTACCACTACACAAAAAAGACCTCAACAAAAATAACCCCATTGCCAGG364840.1336741210030718No Hit
GTTTACCACTACACAAAAAAGAAGTCACCAAAAATAACCCCATTGCCAGG358230.13125227597557945No Hit
GTTTACCACTACACAAAAAAGAACTCACCAAAAATAACCCCATTGCGAAG346700.1270277868429037No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCACAATGCCAAG344790.1263279798833711No Hit
GTTTACCAATACACAAAAAAGAACTCAACAAAAATAACCACATTGCCAAG342910.1256391646561872No Hit
GTTTACCACTACACAAAAAATAACTCAACAAAAATAACCCCATTGCCAGG336340.12323197527182646No Hit
GTTTACCACTACACAAAAAAGAAGTCACCAAAAATAACCCCATTGCCAAG334820.12267506083282671No Hit
GTTTACCACTACACAAAAAAGACCTAAACAAAAATAACCCCATTGCCAAG334270.1224735457397676No Hit
GTTTACCACTACACAAAAAATACCTCAACAAAAATAACCACATTGCCAAG325820.11937754112822292No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCCCATTGCCAAG319200.1169520321899477No Hit
GTTTACCACTACACAAAAAAGACGTCAACAAAAATAACCCCATTGCCAAG307570.11269090394944302No Hit
GTTTACCACTACACAAAAAATACCTCACCAAAAATAACCACATTGCCAGG306790.11240511890837734No Hit
GTTTACCACTACACAAAAAAGAACTCAACAAAAATAACCACAATGCCAAG292330.10710710391631394No Hit
GTTTACCAATACACAAAAAAGAACTAAACAAAAATAACCCCATTGCCAAG287820.10545468015322915No Hit
GTTTACCACTACACAAAAAAGAACTAACCAAAAATAACCACATTGCCAAG281750.10323068630801303No Hit
GTTTACCACTACACAAAAAATACGTCACCAAAAATAACCACATTGCCAAG279560.10242828984655945No Hit
GTTTACCACTACACAAAAAATAACTAACCAAAAATAACCCCATTGCCAAG277590.10170649942232951No Hit
GTTTACCACTACACAAAAAATAACTAAACAAAAATAACCACAATGCCAAG276460.10129247750386258No Hit
GTTTACCACTACACAAAAAAGACGTCACCAACAATAACCCCATTGCCAGG275270.1008564721206983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)