FastQCFastQC Report
Wed 6 Jul 2022
EGAF00006216643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12098593
Sequences flagged as poor quality0
Sequence length90
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2067311.708719352737959No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2009541.660969998742829No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2004391.6567133054232008No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1243191.0275492365103942No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1237811.0231024384405691No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1153890.9537390008904341No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA881060.728233440037201No Hit
TTCTTTGCCTTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA865810.7156286685567487No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA849100.7018171451837416No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA825210.68207104743502No Hit
ACGTTGTGGCCTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA811940.671102829891046No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740930.6124100546237071No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA724440.5987803705769753No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA716920.592564771787926No Hit
TTCTTTGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650640.5377815420355078No Hit
TTCTTTGCCTTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA643500.5318800293554796No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA638660.5278795641774213No Hit
TTCTTTGCCTTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA597390.49376815965294474No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA589810.4875029683203658No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570350.4714184533689165No Hit
GAGTCACCAATCTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570160.47126141031440594No Hit
GAGTCACCAATCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA492970.4074606030635132No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA469130.38775583243439965No Hit
GAGTCACCAATCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA453000.37442370364884575No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA379690.313829880879537No Hit
CCTATTCCGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA368910.30491975389204345No Hit
GCAGAAATCTCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA357910.2958277875782746No Hit
GCAGAAATCTCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA348770.2882731901139248No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA341740.28246259709703436No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT314420.2598814589432011No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA308450.25494700086200106No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG291230.24071394086899195No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT284340.235019063787004No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA283830.234597527167002No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG283310.23416772512307835No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT281630.2327791339042482No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG280030.2314566660767909No Hit
TTCCGAGGATGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250020.20665212888804507No Hit
GCAGAAATCTCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA245400.20283350303626213No Hit
ACGTTGTGGCCTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA239960.19833711242290736No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA219670.18156656728596457No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA206060.17031732532865598No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG203310.16804433375021377No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA201400.16646563778118662No Hit
AACCCACCGTTGTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190860.1577538809678117No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT188510.1558115063462338No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT169470.14007413919949202No Hit
CCGTGTTCCTCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167590.13852023950222972No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA167300.1382805422085031No Hit
GCATTCTGTCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA166150.13733001845751816No Hit
TTCCGAGGATGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160180.13239556037631814No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT160020.1322633135935724No Hit
AACCCACCGTTGTTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA158830.13127972814690106No Hit
TTCTTTGCCTTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG157700.13034573524375934No Hit
GTCTCTTGGCTTAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA157680.1303292043959161No Hit
ACGTTGTGGCCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155370.12841989147002467No Hit
ACGTTGTGGCCTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG154870.12800662027394424No Hit
ACGTTGTGGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154660.1278330463715905No Hit
AATAGCGAGCAAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA152890.12637006633746586No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT148160.12246052082254524No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG146290.12091488654920451No Hit
AATAGCGAGCAAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA145750.12046855365743768No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG143170.1183360742856628No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA143130.11830301258997637No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG140970.11651768102290902No Hit
AACCCACCGTTGTTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135240.1117815931158276No Hit
GTCTCTTGGCTTAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135180.11173200057229796No Hit
TTCTTTGCCTTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT134800.11141791446327685No Hit
CCGTGTTCCTCATTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT134320.11102117411503966No Hit
ACGTTGTGGCCTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG133030.10995493442915222No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA132900.10984748391817131No Hit
TTCTTTGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG131050.10831838049267382No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT130480.10784725132914215No Hit
CCGTGTTCCTCATTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT129900.10736785674168889No Hit
ACGTTGTGGCCTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA129460.10700417808913813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129220.10680580791501955No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT127400.10530150076128687No Hit
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG125990.10413607598834013No Hit
CCGTGTTCCTCATTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC123850.102367275269116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)