FastQCFastQC Report
Tue 12 Jul 2022
EGAF00006216650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006216650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41088828
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGCGTGAGCGAACTGCTCGTAGCG4200641.0223314230330445No Hit
ATAGATGCACAGGAATTGACGCTCATGG4057100.9873973528765533No Hit
TACCCGGCATTTGCTCTCATATGGCTCT3911710.9520130386780562No Hit
TAGAGCCGTACGGTACTTGATGCTCCAC3849190.9367972238098395No Hit
TATGACTCAATGCGCTAGGACGGAGGTT3797240.9241538843600017No Hit
TGATGACTCGTTTCGCCCTAACATGCTT3750460.9127687944762016No Hit
GTCCATTGTCTTTGACATAATCTATTAT3746320.9117612213227402No Hit
GCCCTCATCTCGCCTGTGTGGTATTAGG3736290.9093201684895953No Hit
GAAGGCCCAAGCCACTTTGCTGATAATA3584020.8722614332051525No Hit
ATCGAGGCAATTAGCTATGTGATCGTAT3504920.8530104582199327No Hit
CTCATGCTCCTGTTCAGCTTCTATCATA3488140.8489266230713614No Hit
GCCAACATCAGCTAACGATTTCAGAAAA3425660.8337205432094583No Hit
GGCTTAAGTTATCCTACACATCAGGGTA3421530.832715403807575No Hit
GCACTTACACAAGCCTGGGTCATTTATG3360010.8177429640971994No Hit
CAGCCTTTCCAACTGGCATTACGCAGGC3354730.8164579432637992No Hit
GAGGCTACAAGTGAACACCTAGCGTTCG3344720.814021757933811No Hit
CGACCTGCAGGCTGTTCTCATTCTTCAT3299060.8029092482267929No Hit
AGATGAAGTAACCAGCTGTAATGCTCAC3266270.794928976801188No Hit
AGCGCCTAGTCATGCGGCCCTCGTTGGT3265210.7946709991338764No Hit
AGAAAGGCAGGCAAGCTCTGTCAACGTC3135560.7631174099197963No Hit
GGCAATCGTTTGTGGAATCTCTCCGGTG3135360.763068734888228No Hit
GGAACGGTCCCTCAACGGATCATGCATG3132260.7623142718989211No Hit
TCCGCCATCCACCTGTTACTTACGCATT3123480.7601774380130775No Hit
AATAACCGTCATGCCCGAGTAATTGCTA3113610.7577753252051872No Hit
TGCAAGCGTACGTTTCCTTTCCCCCGAG3092160.7525549280694985No Hit
GGTCAGGAGCACTAACTTCCTTACGACA3092100.7525403255600281No Hit
ACCGGTTCAACCCTCCCGAAAATACACA3063930.7456844473636484No Hit
GCTCACAAGCCTAATATTATTTTCTTAG3053700.7431947194989353No Hit
GTAGCCATCCTTAGTTCGCAGTTTCTGG2986490.7268374751404445No Hit
TGTGCGGGTTCCTCCTGTCGTGCCGGCT2971590.7232111852886142No Hit
ACTCGCTTCATGGCCAAGTTTAATGTAC2950010.7179591493824063No Hit
TTGCCCGTCTGTGAGTTAACGGATATGA2920970.7108915347987049No Hit
CAACCCGCATTGTGATCACCTGGGTGAA2872820.6991730209486627No Hit
TAATCACCAGGTTTGCGTACCATAGTAT2836430.6903165989548303No Hit
TTAAGCCAGGAACCGGAGTGTAGGTGGA2835080.689988042491745No Hit
GGAACGGTTTTTTTTTTTTTTTTTTTTT2817370.6856778684463816No Hit
TTTCACCCAGAAACGTGTGGCTACTTTT2809010.6836432521268312No Hit
TGCTTTAGTGCTGTAAACGACAATCGGC2804040.6824336775923616No Hit
ATATGCATCCTAACGGGTCCATGACAGT2749560.6691745989931862No Hit
AAGCATGAGTTATCCTAAACACACTTCG2721250.6622846482747086No Hit
TAGCGGCTCGCTAGCAATACTCTAATGG2716080.6610263987086709No Hit
ACCACATAGGTGCTGCTTCCCATAGCTG2692870.6553776612951822No Hit
AGTGCCGGTATTGAGTTGTAACGTATGT2671850.6502619154773652No Hit
AGGTGAGGTTGTAAACAATGGCCTGGTT2656300.6464774317729384No Hit
CTTTGGGTTTTTGTTTTAAATTCCTTTT2644790.643676183706189No Hit
TAAGCTATCAATTACGTGTTTATCAGAA2616980.6369079205666319No Hit
GAAGCTAAGTAGAGGCATATTAGGGATG2610530.6353381507985577No Hit
GAGCTTAGTTGCCTCACGAGAAATGGTC2609670.6351288481628145No Hit
AACGACAAGTAACTCATGTACCACCACT2597850.6322521537971343No Hit
CGATCCTTCCAGCACACTCAAACGAGCA2557060.6223248811088017No Hit
GCTATCCTCCTGGCTTCTTTCGGTGGTT2539160.6179684657834486No Hit
GAACCTGTCAAGCTACCCGGGCGGATCA2537650.6176009692951085No Hit
GTATTGCAGGACAACAGGAATCTACTGA2535130.6169876638973494No Hit
TCTTGACGTAACGGGAGAAAATAGTAGT2534230.6167686262552926No Hit
TCCACAACATAGACTTCTGATATCGACC2530830.6159411507186333No Hit
CCAGTTTGTATGGTGCCAAGCCCATGGC2509230.6106842473092686No Hit
CCAGCTGCACTTGTTCTGAACAAAAAAT2483670.6044635782748536No Hit
CGGAGTCTCAATAGCCTCATAGTCTTAG2469110.6009200359766893No Hit
ACATTAGTCACGCGGTTCGTGTCCACTT2444060.5948234882727733No Hit
GTTGCTGTCAGGAAGATAGGCACCGCTT2432910.5921098552628467No Hit
TCCCGGACACGTAAGGAATTTGCTGAAT2400040.5841101138246143No Hit
ACTCAGTAGCATTTCTACCCAGTTTTCT2366830.5760276248327161No Hit
ATTTGTGAGCGGCTGTATGTTTTCTTTG2356890.5736084757637769No Hit
GTGCTTACACCACAACTATCTTAGTTAA2350310.5720070672251835No Hit
GCTAGTGTCGAGGTGGCTAAAACCATCG2349130.5717198845389311No Hit
GTTACTAAGCTTATGAAAGATCATAAAA2341180.5697850520340955No Hit
GCCTTAACACGTGCTGAATTGCTACCAT2300410.5598626468489196No Hit
ATTGCGCCACAACAGGCACGTCAAAGAT2290320.5574069915063044No Hit
TCGATTAAGCTGGCTAGACGATACGTTT2255140.5488450534534595No Hit
CCCAACCGTTAGTGATGTTATTTCCATG2252420.5481830730241319No Hit
TGATGATGTGATTACGAGATCCTGATAG2226550.5418869576907864No Hit
TGAGTGAAGCGAGTAAATGGAATTATTG2209800.5378104237969503No Hit
GTAGTTTCATAATCCGCGCCCAAATATA2186980.5322566026950197No Hit
TCTAAGGGTGCACGCATGGGAAATATCA2182560.5311808844973626No Hit
AATTGCTCATGTCAATGACTAGCCAGTA2171470.5284818539969064No Hit
GTTCATTTCACACAGTGTCGTTGACAGT2166520.5272771469655937No Hit
GCGGGTTTCACAGACTGGCCATTCGAGT2160760.5258753060564297No Hit
CCTATATTCACTAGGTGTATCTAAATGT2132350.5189610178221681No Hit
ATGGTTATCAGCACCACCGGACTTCGAG2130640.51854484630226No Hit
GTGATGAAGTTTGAGCCTTGTACTACAT2130320.518466966251751No Hit
AAGTTAGCAGGTTCACTTTCTAAAGGTT2109890.5134948117770601No Hit
TTGTAAGTCGAGCAAATTGAGTTCTAAA2109260.5133414854276204No Hit
TCATGTTTCCGCAAATTCATGGTACGCT2106480.5126649024888225No Hit
AAAGCCGCAATTGCGCACCTAATTTGCA2030020.4940564379203028No Hit
TCCTGGTTCTTGAGCGTGAATCTAAATA2029330.4938885090613926No Hit
GCGAAGTAGTTCCTGCAATGCATTTTAC2005670.4881302528268755No Hit
CATAGCTAGTTATCTCACGTACAGTCTT2003790.4876727075301345No Hit
ACACTAATCCGGAACCAGCATCACTGTA1980290.48195339132087195No Hit
CTGTGTGAGGATTGCTACCTCTCGTCGT1951000.4748249329477102No Hit
CCATCACTCTTTAGGAGCCGTCGGCGCA1944910.4733427782364588No Hit
AGACACGCAAACGGGCCTCTTCCACCAT1910830.4650485528572389No Hit
TGGGCCTAGCATGTTAGTTAAATGTACC1902630.4630528765629431No Hit
TCACATAAGTTGTCCCTTGCTGAATTTT1896910.4616607706600928No Hit
GGGTGAAGTAGGTGTCGGAAGGTGGCGC1888760.459677263123689No Hit
TGATTCAAGCGGATTTGAGTCGATCTAT1886430.45911019900591954No Hit
GCCTGTGCAATGAAGCAAGGCCCTTATA1881030.4577959731535784No Hit
GTGAGGAGTAAACAAGGACCCTATGTGT1872260.45566157301931315No Hit
CCTTACTCAATCCCTTTTACCACAGCCT1869560.45500446009314255No Hit
AATCTCAAGAGAGGCTGCATGTATAGGA1860930.4529041324809751No Hit
AGTAACGAGGTACCGCGCTTTTGGCTGC1848000.44975729169009154No Hit
GGTTTCCTCAGCTAACGAGGCCACAATA1829590.44527675503423947No Hit
GCTTACCTCACTTTACGATCCTTCTTCA1821670.44334922378413905No Hit
GGGCATGAGCCTGTTCGACCAGCATACA1817690.4423805906559321No Hit
TATCCAGCATCAGCACAGTTTGTTTAAA1789290.4354687361732488No Hit
GTCTAATCAAATATCCTAGGTTCCCCCC1748180.425463583434407No Hit
GTCTAATCAAATATCCAGACTGATTGAG1745040.42469938543878644No Hit
CGTTAAATCTGTGCCTGTTAGTACTAGG1744480.4245630953503955No Hit
TACTGCACATTGCAGCTCAGGACTCATC1739260.42329267702646567No Hit
TGAGGCACATAAGTCTCCTTTTTTGTTT1723100.4193597344757558No Hit
TCTAGCACAGCAGGTAAAAACTTTACAG1722710.41926481816419786No Hit
CGACTAAGTCACGGATATATCTAAACTT1717810.4180722798907771No Hit
AGTAACCTCTGTGCAGATTTTACTCTAT1680790.4090625315475048No Hit
TGTTGTAAGCTTAACAAGAATCAGGATC1668980.4061882709334031No Hit
GGCATTAGTTGTTGCTGGGTAAAACTGA1663570.4048716113294835No Hit
CTAATTGAGGCGAAACCTTTTTTGTTTT1659170.40380076063498327No Hit
TTGAGCTAGTTCCCACTGTCATTTCTGG1652120.40208496577220454No Hit
GAATTTGTCCATAAGCTTCATCCCTAAT1649540.40145705786497493No Hit
TTTGTCCCAGTAGGTGTTTGTAATAGTC1616690.3934621839298994No Hit
TAGGTTGGTACCTTACGCAGGGTGAAAG1601180.3896874352317861No Hit
CGAAGGCCAAGCCACTTAATTTCGTCAG1595270.38824908804894603No Hit
CTTTCTTGTTCAAGATGTTGCAGGCACG1565270.3809478333137173No Hit
CAAAGTGAGTTAGGCTTTTGCTTCTGGC1559150.37945837734773064No Hit
GAAGCCTGTTAGCTGACTTTAGTAATTA1510880.36771065847874757No Hit
GAGCATGCAGGTCCTGATTCCGGTAGGA1497250.36439345507737525No Hit
GCCACTAAGTTGGCCATCGATATTGTCA1470680.35792697713354105No Hit
GAGCTTGTCCAATAGCTGTTCAGGGTTA1421900.3460551369340591No Hit
TGCCGGTAGCCTTAAACGTTCACGCCTC1414370.3442225219955166No Hit
ACTCAGTAGCATTTCTGTAATTACACTG1400510.3408493423078409No Hit
TGTTACTTCAATCTAGTACACGACTCAT1382670.3365075294919583No Hit
AATGTCATCACTTTACATAGAACAGGTG1330550.32382281626528747No Hit
TGTGGCGGTAGTAAGACTTTTAACATGT1318690.32093638689329373No Hit
TGAGCCGGTGAATCGCGGCTTCGTCCGG1309000.31857808161381485No Hit
AGTGCACGTCTTTATGAGGCAGCACAGC1304450.31747072464563847No Hit
TTGTCCGGTGCTGGTGTTTTCTCGGCGG1290400.314051303677973No Hit
GTTAGACTCCAAACACTGGATTTCCGTT1249110.30400234341071986No Hit
AGGCAGGTCCTGGCTTCGATATTCTATT1244180.3028025038825639No Hit
CTCTCACAGTGCACGCACGGTTAGGTCG1222520.2975309979637287No Hit
GGTTGAGCAGCACGTTCTCTTTGCTAAT1201940.2925223372153618No Hit
GAGGTACAGGTTACACGGCATGCTGATC1176060.2862237881304378No Hit
CCTTCAATCGGTCATGAGGCGTAAAGTG1147430.27925595736145115No Hit
GATTATGTCTCATCATCGAATACGTCTG1146360.2789955459425613No Hit
CCTGTAACATCCCTCATGTCCATTAGTA1134130.2760190677621664No Hit
GGCTAGACACCGTTCCTGCCGTTATCGG1133990.27598499524006864No Hit
AGCCTCCGTCCTTCAGATCATGGAGTTG1117910.2720715227019861No Hit
GCGCAAACAGGCCTTGCGCGCTAGAATT1059700.2579046547640638No Hit
AGCATCCCAATATACCTAGGGAATTTGT1058900.25770995463779106No Hit
TTGGATCAGTGAACAGCCAGTAACTGGT1058090.25751282075993986No Hit
TTGGATATCCGGTTAGTCCTGCCTCCAT1005400.24468938369329982No Hit
TCAAGTATCTTACTCGCTTTGAACAGAC1003100.2441296208302656No Hit
ACTAAAGCAACTCGCGTATTATTATCTT966040.23511013748067966No Hit
TGTGCGGGTGCAATATAAAATAGTAATG963230.23442625328714659No Hit
CTTACCGGTGGGAACAGACAATGTTAAA936850.22800601662330208No Hit
AAGTTAGCAACTAGAACGTAAACCAGTT868790.21144190338064645No Hit
CATCACACAAGTAAGCATGTTATACAGT843560.20530154814831905No Hit
TGTATCGCAGGTATTTCATCATATTCCT842570.2050606067420565No Hit
CGATCCTTCACAGACTTTTGCACATAGT835640.20337401689821868No Hit
CCTACTTCATGTCAGCCCTCGTTCAGCT827550.20140511187128532No Hit
AGGAACCAGCACAGAACATTTCATTTAC820940.19979640207795657No Hit
CTACCTCAGTAGAGGCTATGCTTTAAGT817720.19901273406970868No Hit
CGGGCTTAGGGATGCGACAATATCTAAA814930.1983337173793324No Hit
ACCCAGGGTACAATGTTTTCCAGACTCT790850.19247324357852214No Hit
TACTAAGTCATCCTGCAAAGTCAGAATT781080.19009546828641596No Hit
TCGTAATCAATTAAGGTCTACGCTGGGC760520.18509167504120586No Hit
CTTATGTTCACTCGCTAGTTGTGACGGG744920.18129502257888688No Hit
CTTGTAAAGTTAGTGCGTCATATAATTG733930.17862032959421476No Hit
TGTGATCAGGTCCAATCATCAACTAGAT732110.17817738680694423No Hit
GTCTATGCATGACTATAACTTCCAAGCC731910.178128711775376No Hit
CGATCCTTCTCCATATGTCAACGCCCGA720120.17525931866443112No Hit
CATGGATTCATAGACCTACTTTCGCAGC714110.17379663396580697No Hit
GTTGCGGGTTAAGTGTGAGTAGCAGGGG704150.171372617393711No Hit
GTGGCTTCATACCCCGGAAACTAGTGTG699140.1701533078529278No Hit
TAGGTGCGTGTGAGGATAAGTTTTGTGG694460.16901431211423212No Hit
GCCAGGTTCCAGGTCACCCAAGAACAGT684770.16665600683475323No Hit
TGTTGCACAATTAGCTACGATAAAGGGA680770.1656825062033894No Hit
ACGTTGTAGGCCTAATATTCTTACATTA634000.15429985007116778No Hit
CTGTTTAGTGTTAAACTCTGAGTCCGAT609910.14843694251877906No Hit
AACGGTAAGTCCTGAGCGCTATCTACGA600150.14606160097825133No Hit
GTGCAAGCATGCTCCCACGTATTGTATT594900.14478388139958628No Hit
ACTTGTCGTTACTTGCTATATTTAGGGC588530.14323358164413938No Hit
CGTTAACAGCTGCACATTTCTCAGTACG581260.14146424424663562No Hit
TTCAACCGTTTATCTGACACTTTATGAG570250.13878468375880665No Hit
TTTAGCTTCCAAGTTACGTCCGTCACAG561090.13655536731298346No Hit
CTAATCTTCAATAGCCATCCGCTCCTGC558820.13600290570468448No Hit
GGAAACTCACTTAGGCAAACTAAATTCC554250.13489068123335132No Hit
TTACCTGTCGGTTCCTTTCAGTGCCTTG542460.1320212881224064No Hit
AAAGCACCACCTGGTGATACATCTTCTT534240.13002074432495375No Hit
GCGAGTGAGCACTAACTTCTATCCCATG530210.12903994243885467No Hit
CTGATCACACGAATCCCTACTCAGCGTG522570.12718055623294974No Hit
GAGGACTAGCTAGAAGTCGCTAGATGTG508010.12363701393478539No Hit
ACACTTAAGTACTGGTCTATGCGGGAAT504400.12275842961497953No Hit
TCTATGTTCCCGCCTAACATCCAGTCAA495650.12062889698387114No Hit
CGCAAATTCTTAATGGACAATTCTACAC492450.11985009647878007No Hit
TTCAGCACAGGTCCTGAGAAATTTCTAG492420.11984279522404484No Hit
GCTGGATGTCGTAAATTATGGCTCTCTT476590.11599016647542246No Hit
TATTCGTTCAAATCGCACAGCTAATATT467680.11382169381905953No Hit
AGGATTGAGGAGTCTTAAATTAGTGAGG460460.1120645251794478No Hit
ATTTGCGCAGGCTAGAGGCATATGTTGG457910.11144391852695337No Hit
CATTATCTCCACCTGTTGCCTTAGGAGT457410.11132223094803288No Hit
TACTCAAAGTCACCTCACTTTTAGATGT445670.10846500659498003No Hit
TTCGCAACAAACTCATTGTGATATACAC438140.10663239165643762No Hit
GACACCGGTTATCGAGCTGTACGGGCTT436370.10620161762705911No Hit
TTCCTAATCTTGCTATAACTATAATCAT432970.10537414209039984No Hit
GCTTTCATCCGGCTAACTTCAGAGGACT432270.10520377947991118No Hit
GTCTAGCCAAGGACCATCACGTTGAGGA414620.10090820794401827No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)